目的:观察额尔敦-乌日勒对动脉粥样硬化消退的影响,并检测相关炎症因子,探讨其作用机制。方法:用高胆固醇喂养方法复制家兔动脉粥样硬化模型,并予额尔敦-乌日勒干预治疗12w。于实验结束时检测血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HLD-C)和血清中炎性标志物高敏C反应蛋白(hs-PCR)含量,以及血管细胞粘附分子-1(VCAM-1)的水平;观察各组家兔主动脉动脉粥样硬化情况;检测主动脉中VCAM-1 mRNA表达水平。结果:与自然消退组比较,额尔敦-乌日勒治疗组的血清TC、TG、LDL-C水平降低,HLD-C变化不明显;主动脉斑块面积占总面积的百分比及内膜/中膜厚度比减小;血清中hs-PCR含量和VCAM-1水平降低,主动脉VCAM-1mRNA表达水平下降。结论:额尔敦-乌日勒对早期动脉粥样硬化具有消退作用,其机制可能与降脂、抗炎有关。

The study is aimed to observe the effect of Eerdun-wurili on atherosclerosis recession.Related inflammation factors were detected in the mechanism discussion.High cholesterol feeding method was used in the rabbit atherosclerosis model establishment.Eerdun-wurili was used in a 12-week treatment.Content of serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HLD-C),and serum inflammatory markers hypersensitive C-reactive protein(hs-PCR) were detected after the experiment.Level of vascular cellular adhesive molecular-1(VCAM-1) was also tested.The aortic atherosclerosis severity was observed.The VCAM-1 mRNA expression level in the aorta was also observed.The results showed that compared with natural recession group,level of serum TC,TG,LDL-C in the treatment group were reduced.The content change of HLD-C was not significant.The percentage of aortic plaque area in total area and the ratio between inner-membrane and tunica media were also decreased.Serum hs-PCR content and VCAM-1 level were reduced.VCAM-1mRNA aortic expression level was also declined.It is concluded that Eerdun-wurili plays a recession role in early atherosclerosis.Its mechanism may be related to cholesterol-lowering and anti-inflammation.

国家自然基金委地区科学基金项目(30860386):蒙药额尔敦-乌日勒抗动脉粥样硬化的分子机制研究,负责人:麻春杰