目的：建立以高效液相色谱法测定异长春花碱脂质体中主药含量的方法。方法：色谱柱为Kromasil C18（4.6 mm×250 mm，5 μm），流动相为乙腈-0.06 mol·L-1 磷酸二氢钾缓冲液（pH3.0）（35︰65），流速为1.0 mL·min-1，检测波长为215 nm，柱温为30益。结果：异长春花碱检测浓度的线性范围为2.08~20.8 μg·mL-1（r=0.9999）。平均加样回收率为98.64%（RSD=1.09%）。结论：本方法简便、准确、重现性好，可用于该制剂的含量测定。

The study is aimed to establish a method to determine the content of Vinorelbine in liposomes by HPLC.The experiment was carried out on Kromasil C18 (4.6 mm×250 mm, 5μm) column with the mobile phase consisted of acetonitrile-0.06 mol窑L-1 KDP buffer (pH 3.0, 35∶65) at a flow rate of 1.0 mL?min-1. The detection wavelength was at 215 nm and the column temperature was at 30℃. The result showed that the linear range of Vinorelbine was from 2.08μg?mL-1 to 20.8μg?mL-1 (r=0.9999). The average recovery rate was 98.64% (RSD =1.09%). It is concluded that the method used in this analysis is simple, precise and replicable with high recovery and accuracy. It can be used to determine the content of total Vinorelbine in liposomes.

国家自然科学基金项目（81102822）：基于IMC介导pNP-PEG修饰肿瘤血管亲和的荷电脂质体及协同靶向NSCLC机理研究，负责人：李学涛。