目的：研究不同地区大蓟炒炭后止血药效和成分的差异。方法：昆明小鼠随机分成阳性对照组、空白组、樟树大蓟炭组、安徽大蓟炭组、湖南大蓟炭组、河北大蓟炭组。连续给药3天后，采用小鼠断尾法和玻片法分别测量各组小鼠尾部出血时间及凝血时间，并用HPLC法初步比较不同地区大蓟炭的成分差异。结果：樟树、湖南地区大蓟止血效果要优于河北地区产大蓟，安徽地区大蓟没有止血效果，且呈现一定的促进出血效果。HPLC图谱显示不同地区产大蓟虽经鉴定均为正品大蓟，但成分差异较大。结论：不同产地大蓟止血效果、成分均存在显著差异，为进一步揭示大蓟炒炭止血增效提供了科学依据。

This study was aimed to evaluate the difference of hemostatic effect and components of Carbonized Cirsium japonicum DC from different regions. Kunming mice, half male and half female, were randomly divided into six groups, which are the positive control group, blank control group, Zhangshu Carbonized C. japonicum DC group, Anhui Carbonized C. japonicum DC group, Hunan Carbonized C. japonicum DC group, and Hebei Carbonized C. japonicum DC group. Each group received an oral administration for three consecutive days. An hour after the last oral administration, the bleeding time and coagulation time of each mouse was checked. High pressure liquid chromatography (HPLC) was used to study the difference of compounds in C. japonicum DC from different regions. The results showed that Carbonized C. japonicum DC from Zhangshu and Hunan has stronger hemostatic effect than Carbonized C. japonicum DC from Hebei. The C. japonicum DC from Anhui showed no hemostatic effect and even promoted the bleeding. It was concluded that there were differences of hemostatic effect and compounds of Carbonized C. japonicum DC from different regions.

国家自然基金项目（81060343）：大蓟炭止血药效物质基础及增效作用机制研究，负责人：龚千锋；江西省科技厅对外科技合作项目（赣财教[2008]212）：大蓟炭止血药效物质基础及增效作用机制研究，负责人：钟凌云。