目的：建立高效液相色谱-蒸发光散射检测（HPLC-ELSD）法测定新生脉散液中黄芪甲苷含量。方法：色谱柱：Waters SunfireTM C18（4.6 mm×250 mm，5.0 μm）；流动相：乙腈-水（35∶65）；流速：1 mL·min-1；柱温：35℃；蒸发光散射检测器参数：漂移管温度100℃，载气流速2.6 mL·min-1。结果：线性范围为0.024~0.240 mg·mL-1 （r=0.9997），平均回收率为99.09%，RSD为1.55%（n=6），重复性RSD为1.22%（n=6），精密度RSD为0.64%（n=6），稳定性RSD为1.61%。结论：本方法操作简便，准确灵敏，可用于新生脉散液的质量控制。

This study was aimed to measure astragoloside Ⅳ content in New Shengmaisan Liquid using HPLCELSD method. Astragoloside Ⅳ were assayed on a Waters SunfireTM C18 (4.6 mm×250 mm, 5.0 μm) column, with the mobile phase composed of Acetonitrile-Water (35: 65). The flow rate was 1 mL/min and the column temperature was 35℃. The drift tube had a temperature of 100℃, with the gas flow being set at 2.6 mL/min. The results showed that the calibration curves were linear ranging from 0.024 to 0.240 mg/mL (r = 0.9997) for astragoloside Ⅳ. The average recovery (n = 6) of astragoloside Ⅳ was 99.09% (RSD = 1.55%). It was concluded that this method is simple, accurate, sensitive and can be used for the quality control of New Shengmaisan Liquid by HPLC-ELSD.

科学技术部国家重大新药创制专项（2010ZX09102-202）：治疗心力衰竭（气虚血瘀水停证）中药新生脉散片临床前研究，负责人：毛静远。