目的：建立维吾尔药材香青兰的DNA 条形码鉴别方法。方法：采用ITS2 序列对香青兰Dracocephalum moldavica L. 及其10 种近缘种的25 份样品进行PCR 扩增并测序，比较种内和种间变异，采用K2P 模型构建NJ 系统树，并比较二级结构差异。结果：产自新疆的香青兰（KF041160、KF041163、KF041168、KF041169）种内无变异，来源于GenBank 的香青兰（AY506659）种内存在2 个变异位点；NJ 树显示香青兰可以与其近缘种区分开；垂花青兰D. nutans L.、羽叶青兰D. bipinnatum Rupr.、全叶青兰D. integrifolium Bge.等也可以与其他近缘种区分。结论：ITS2 条形码序列可以鉴定香青兰及其近缘种植物，提供了维吾尔药材香青兰分子鉴定的有效途径。

Objective: To establish the DNA barcode identification method of Uygur Medicine Dracocephalum moldavica L. Methods: D. moldavica L. and its ten sibling species of twenty five samples was amplified by ITS2 sequence, after sequencing and comparing the intraspecific and interspecific variation, we using K2P and NJ methods to analysis the their relationship, then compare the secondary structure. Results: D. Moldavica (KF041160, KF041163, KF041168, KF041169) from Xinjiang without variation in intraspecies, but there are two 2 variation sites in D. moldavica (AY506659) from GenBank. By NJ method, D. moldavica can be distinguished with their sibling species. Also, D. nutans L., D. bipinnatum Rupr. and D. integrifolium Bge. can be distinguished with other sibling species. Conclusion: ITS2 barcode sequence was able to identify D. moldavica and its sibling species, which provides an effective way for the molecular identification of Uygur Medicine D. moldavica.

新疆维吾尔自治区科技厅科技基础条件平台建设项目（PT1211）：新疆传统药物DNA 条形码鉴定技术平台建设，负责人：李晓瑾；乌鲁木齐市科技厅科技攻关项目（G121120004）：新疆民族药材鉴定DNA 条形码数据库建设与关键技术研究，负责人：李晓瑾。