目的：通过丹花颗粒对急性心肌缺血大鼠炎症因子表达的影响，探讨其保护心肌缺血的作用机制，为进一步研究奠定基础。方法：120 只Wistar 大鼠，按照随机数字表法分为假手术组、模型组、丹花颗粒高、中、低剂量组、通心络组。灌胃14 d 后，以结扎冠脉左前降支建立急性心肌缺血模型。全自动生化仪检测谷草转氨酶（AST）、乳酸脱氢酶（LDH）、肌酸酶同工酶（CK-MB）；记录造模前后ST 段变化；双抗夹心酶联免疫吸附方法（ELISA）检测白介素-6（IL-6）、肿瘤坏死因子-ɑ（TNF-ɑ）、血栓素（TXA2）、前列环素（PGI2）、血清高敏c 反应蛋白（CRP）、肌钙蛋白I（Tn-I）浓度；免疫组化方法检测心肌细胞NF-κB 表达。结果：造模前后治疗组与模型组比较ΣST 变化显著（P＜0.01）。与模型组比较，丹花颗粒高剂量组与通心络组AST、LDH、CK-MB、Tn-I 浓度均明显降低（P＜0.05），低剂量组与中剂量组CK-MB、Tn-I 浓度明显降低（P＜0.05），丹花颗粒组与通心络组CRP 表达明显减少（P＜0.05），丹花颗粒组与通心络组IL-6、TNF-ɑ浓度明显降低（P＜0.05），丹花颗粒组与通心络组PGI2 水平均明显增加（P＜0.05）；TXA2 水平明显降低（P＜0.05）。丹花颗粒组及通心络组NF-κBp65 阳性表达明显减弱（P＜0.05）。结论：丹花颗粒可能通过抑制NF-κB 细胞外的正反馈激活途径，从而减少IL-6、TNF-ɑ、TXA2、PGI2、CRP 等炎症因子的基因转录，阻断后者所介导的一系列炎性反应，可能是其防治心肌缺血的作用机制之一。

This study was aimed to explore mechanism of Danhua particles in the protection of myocardial ischemia, and lay the foundation for further study through the expression of inflammatory cytokines of Danhua particles in rats with acute myocardial ischemia. A total of 120 Wistar rats were divided into the sham group,model group, Danhua particles high-dose group, middle-dose group, low-dose group, Tongxinluo group, according to the random number table. The automatic biochemical analyzer was used in the detection of the concentration of the AST, LDH, CK -MB. And the ECG was used to record the ST -segment changes before and after modeling. The ELISA kit was used in the detection of concentration of IL-6, TNF-ɑ, TXA2, PGI2, CRP and TN -I. Immunohistochemical methods were used to detect myocardial cell NF -κB expression. The results showed that before and after the modeling, the treatment group compared with model group ΣST changed significantly (P < 0.01). Compared with the model group, the Danhua particles high -dose group and Tongxinluo group were significantly reduced in the concentration of AST, LDH, CK -MB and Tn -I (P <0.05); the low-dose group and middle-dose group were significantly reduced in the concentration of CK-MBand Tn -I (P < 0.05); the expression of CRP in the Danhua particles group and Tongxinluo group was significantly reduced (P < 0.05). Danhua particles group and Tongxinluo group compared with the model group were significantly reduced in the concentration of IL-6 and TNF-ɑ(P < 0.05); the PGI2 level in the Danhua particles group and Tongxinluo group was significantly increased (P < 0.05); and the TXA2 level was obviously reduced (P < 0.05). The positive expression of NF -κBp65 in the Danhua particles group and Tongxinluo group was significantly reduced (P < 0.05). It was concluded that Danhua particles inhibited the NF-κB extra cellular positive feedback activation pathway, and thus reduced the inflammatory factor gene transcription such as IL-6, TNF-ɑ, TXA2, PGI2, CRP, block a series of inflammatory reactions which were mediated. It may be one of the mechanisms of Danhua particles in the prevention on myocardial ischemia.

吉林省科技厅科研专项（20120936）：中药丹花颗粒临床前研究，负责人：邓悦。