[关键词]
[摘要]
采用营养液培养法,研究苯甲酸、邻苯二甲酸二异丁酯、丁二酸二异丁酯、棕榈酸、2,2-二(4-羟基苯基)丙烷胁迫对人参根系分泌物组成的影响。GC-MS 分析发现,包括对照在内的6 个处理中分别检测到15~23 种化合物,包括有机酸类、醇类、氨基酸酯类、酚酸类、胺类、稠环类、糖类等。与对照比较发现,胁迫处理后大部分人参根系分泌液中酚及酚酸类物质种类有不同程度增加。由此推测,释放到土壤中的自毒物质会刺激人参根向外界释放自毒物质,进而加剧对人参的毒害作用。
[Key word]
[Abstract]
By nutrient solution culture method, effects of benzoic acid, diisobutyl phthalate, diisobutyl succinate,palmitic acid, 2,2-bis(4-hydroxyphenyl) propane on root exudates of Panax ginseng were studied. GC-MS results indicated that, 15 to 23 components were detected in treatments including control, which were organic acids, alcohol,amino acid esters, phenols, amines, condensed nucleus, saccharine, etc. Through compared with control we found that, phenol and phenolic acids in root exudates increased in most of stress treatments. So we deduced that, the relaxation of autotoxins from roots to outside environment was stimulated by autotoxins released into soil, and which accelerated the autotoxicity to ginseng seedlings.
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[基金项目]
国家自然科学基金委面上项目(81072992):自毒物质对人参根际微生物区系影响及其解毒细菌的研究,负责人:丁万隆;国家工业与信息化部中药材生产扶持项目[工信部消费(2011)340]:中药材规范化生产技术服务平台,负责人:丁万隆。