目的：建立HPLC 测定广西不同产地龙眼花药材中槲皮素、木犀草素和山奈素含量的方法。方法：Hypersil C18 色谱柱（4.6 mmx250 mm，5 μm），流动相为甲醇-0.2%磷酸（47:53），流速1.0 mL·min-1，柱温30℃，检测波长360 nm。结果：槲皮素、木犀草素和山柰素分别在0.18~2.88 μg、0.059~0.944 μg 和0.024~0.384 μg 之间呈良好的线性关系（r=0.999 9），平均加样回收率分别为100.06%、99.77%、98.67%，RSD 分别为1.72%、1.18%、1.99%（n=9）。结论：该方法简便可靠，快速，重复性好，可用于龙眼花药材的质 量控制。

This study was aimed to create a method for content determination of quercetin, luteolin and kaempferol in flowers of Dimocarpus longan Lour. from different habitats of Guangxi province by HPLC. The samples were separated on the Hypersil C18 column (250 mm x4.6 mm, 5 μm) which was eluted with methanol-0.2% phosphoric acid solution (47:53) with detective wavelength at 360 nm, flow rate at 1.0 mL·min-1, and column temperature at 30℃.The results showed that this method had a good linear relationship within the range of 0.18 to 2.88 μg for quercetin,0.059 to 0.944 μg for luteolin, and 0.024 to 0.384 μg for kaempferol (r = 0.999 9). The average recovery rate was 100.06% (RSD = 1.72%), 99.77% (RSD = 1.18%) and 98.67% (RSD = 1.99%, n = 9), respectively. It was concluded that this method is simple, rapid, reliable and with good repeatability, which can be used in the quality control of flowers of Dimocarpus longan Lour.

2009 年度广西高校优秀人才资助计划（[2009]62 号）：龙眼花药材降血糖药效物质基础的研究，负责人：梁洁；广西省自然科学基金委青年基金项目（2010GXNSFB013071）：广西龙眼叶降血糖药效物质基础的研究，负责人：梁洁。