目的：研究治伤巴布剂对大鼠炎性疼痛是否有镇痛作用，探讨治伤巴布剂对福尔马林足底炎性疼痛大鼠模型背根神经节中Nav1.7 水平的影响。方法：SD 大鼠36 只，随机分为3 组，即空白对照组、模型组、治疗组，每组12 只，空白对照组大鼠不予任何处理，模型组左后足底注射5%福尔马林0.1 mL，治疗组大鼠造模前1 天于左后足外敷治伤巴布剂，后左后足底注射5%福尔马林0.1 mL，观察造模后大鼠疼痛行为学反应，并以Dubuisson 评分方法进行记录比较。各组大鼠在指定时间点取出L3-6 节段背根神经节，运用实时荧光定量、蛋白质印迹检测Nav1.7 在大鼠背根神经节中的表达水平。结果：治疗组大鼠疼痛反应积分低于模型组（P＜0.05﹚；实时荧光定量结果示，模型组Nav1.7mRNA 相对表达量大于治疗组，治疗组Nav1.7mRNA 相对表达量大于空白组，3 组组间比较差异显著（P＜0.05），3 组组内3 个时相点的相对表达量差异均无统计学意义；蛋白质印迹法灰度扫描示模型组Nav1.7 相对表达量大于治疗组，治疗组Nav1.7 相对表达量大于空白组，3 组组间比较差异显著（P＜0.05），3 组组内3 个时相点的相对表达量差异均无统计学意义。结论：治伤巴布剂能缓解大鼠炎性疼痛反应，对大鼠炎性疼痛具有一定的镇痛作用，并对福尔马林足底炎性疼痛大鼠模型背根神经节中Nav1.7 的表达水平有影响。

This study was aimed to observe the analgesia of curing-injury Cataplasma and discuss the Nav1.7expression in dorsal root ganglion(DRG) in model rats with formaldehyde-induced inflammatory pain. A total of 36 Sprague-Dawley rats were divided into three groups, which were the blank control group (n = 12), model group (n = 12), and treatment group (n = 12). The blank control group was without any treatment. The model group was injected with 0.1 mL 5% saline formalin on the left rear foot. The treatment group was applied with curing-injury Cataplasma on the left rear foot 24 h before the injection of 0.1 mL 5% saline formalin in the establishment of animal model. The behavior reactions to pain of model rats were observed. Dubuisson score was recorded and compared. Meanwhile, L3-6 DRG was collected from rats in each group. The expression of Nav1.7 was detected by real-time quantitative PCR and western blot. The results showed that the pain reaction integral in the treatment group was lower than the model group (P < 0.05). Results from the realtime quantitative PCR showed that the relative expression of Nav1.7 mRNA in the model group was more than the treatment group. And the relative expression of Nav1.7 mRNA in the treatment group was more than the blank control group. There was significant difference among three groups (P < 0.05). There was no statistical difference at the three time points within three groups. Results from the western blot showed that the relative expression of Nav1.7 in the model group was more than the treatment group. And the expression of Nav1.7 in the treatment group was more than the blank control group. There was significant difference among three groups (P < 0.05). There was no statistical difference at the three time points within three groups. It was concluded that the curing-injury Cataplasma can alleviate inflammatory pain response in rats, and have certain analgesia effect. Meanwhile, it can influence Nav1.7 expression in DRG in model rats with formaldehyde-induced inflammatory pain.

湖南常德科技局科研项目（2011sk13）：治伤巴布剂干预大鼠背根神经节钠通道Nav1.7 表达研究，负责人：邵先舫。