目的：建立生脉超微粉中3 种人参皂苷的含量测定方法。方法：采用kromasil C18（250 mmx4.6 mm，5 μm）色谱柱，以乙腈（A）-水（B）为流动相，梯度洗脱0~35 min，19%A；35~55 min，19%~29% A；55~70 min，29%A；70~100 min，29%~40%A,流速1 mL·min-1，检测波长203 nm，柱温30℃，进样量10 μL。结果：人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1 线性范围分别为0.083~0.834 μg、0.086~0.863 μg、0.091~0.911 μg；平均加样回收率（n=6）分别为100.7%、100.5%、100.5%。结论：本方法快速、灵敏，重现性好，适 合于生脉超微粉中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1 的含量测定。

This study was aimed to establish an HPLC method to determine three ginsenosides in Shengmai ultramicro powder. The kromasil C18 (250 mm x4.6 mm, 5 μm) was used as analytical column. The mobile phase was composed of acetonitrile (A) and water (B) with gradient elution (0~35 min, 19% A; 35~55 min, 19%~29% A; 55~ 70 min, 29% A; 70~100 min, 29%~40% A) at a flow rate of 1 mL·min-1. The detection wavelength was 203 nm and the column temperature was 30℃. The injection volume was 10 μL. The results showed that the linear ranges of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 were 0.083~0.834 μg, 0.086~0.863 μg, 0.091~0.911 μg, respectively. The average recovery rates (n = 6) were 100.7%, 100.5%, 100.5%, respectively. It was concluded that this method was quick, sensitive, repeatable and suitable to determine contents of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Shengmai ultra-micro powder.

科学技术部国家重点基础研究发展计划（973 计划）项目（2010CB735604）：新药研制过程化学机理———中药制药过程控制技术模式和方式研究，负责人：萧伟。