目的：克隆刺五加Actin 基因的全长cDNA 序列，并对其进行生物信息学分析。方法：以刺五加的叶片为材料，提取总RNA，逆转录为cDNA，根据初步克隆到的刺五加Actin 基因保守序列设计引物，利用套式PCR 进行3' 和5'RACE 扩增，得到Actin 基因的3' 和5' 端cDNA 序列片段，拼接获得cDNA 全长。将该序列进行BLAST 比对、相似度分析，并对刺五加Actin1 蛋白的二级结构和三级结构进行预测。结果：刺五加的Actin 基因全长1 507 bp，命名为ESActin1，注册号KC469585 。该基因开放阅读框（ORF）长1 134 bp，编码377 个氨基酸，5' 端非编码区长140 bp，3' 端非编码区长233 bp。ESActin1 与GenBank 中注册的其它植物肌动蛋白核苷酸序列的相似性在75%以上，蛋白质序列的相似性在94%以上。结论：首次报道了刺五加Actin 基因的全长cDNA 序列，为刺五加的分子生物学研究奠定基础。

This study was aimed to clone the full-length cDNA sequence of actin gene of Eleutherococcus senticosus. And bioinformatics analysis was used. The total RNA was isolated from leaves of E. senticosus, and cDNA was synthesized by reverse transcription of total RNA. Primers were designed according to the conserved sequence that had been cloned of Actin of E. senticosus. Then, the 3'and 5' cDNA fragments were cloned by nested PCR. The full-length gene was obtained by gene splicing method. Sequencing results were compared and treated with similarity analysis by blast analysis in the GenBank. Protein secondary structure and tertiary structure of Actin of E. senticosus was predicted by online software. The results showed that the full-length cDNA of Actin of E. senticosus is 1507 bp, which named EsActin1, GenBank accession No. KC469585. The conserved sequence, which contained a 1134 bp open reading frame that encoding a 377 amino acid residues, a 5'-UTR of 140 bp and a 3' -UTR of 233 bp. Homologous alignment showed that it shared over 75% nucleotide sequence similarity and over 94% amino acid sequences similarity with Actins in other plants. It was concluded that this study first isolated and reported the full-length cDNA sequence of actin gene of E. senticosus, and laid a foundation for the molecular biology research of E. senticosus .

国家自然科学基金委青年基金项目（30701086）：刺五加苷关键酶基因单核苷酸多态性及其对刺五加苷含量的作用机制，负责人：邢朝斌；河北省自然科学基金项目（C2009001252）：内生菌提高刺五加中药用成分含量的作用机制研究，负责人：邢朝斌；河北省自然科学基金-石药集团医药联合研究基金项目（H2012401006）：刺五加鲨烯合酶基因家族及其对刺五加苷含量的作用机制，负责人：邢朝斌；河北联合大学培养基金（GP201306）：关键酶基因家族对刺五加苷含量的作用机制，负责人：邢朝斌。