目的：探讨金振口服液（JZKFY）对脂多糖（LPS）致急性肺损伤（ALI）大鼠的影响。方法：实验动物随机分为正常组、模型组、阳性对照组、JZKFY 高、中、低3 个剂量（4.4、2.2、1.1 g·kg-1），各给药组连续灌胃给药7 天；末次给药后1 h，除正常组外，每鼠气管内滴注LPS（6 mg·kg-1）复制LPS 致ALI 大鼠模型，正常对照组给予等体积生理盐水，注射LPS 16 h 后麻醉大鼠，取肺，进行组织学观察，并检测肺通透性、肺组织MPO、MDA、SOD 活性、血清TNF-ɑ、IL-6、IL-1β的含量。结果：JZKFY 高、中、低剂量组均能降低肺通透指数；JZKFY 高、中剂量组能调节肺组织MPO、MDA、SOD 活性；JZKFY 高、中剂量组明显降低血清TNF-ɑ、IL-6、IL-1β的含量；JZKFY 高、中剂量组明显改善ALI 的肺组织病变。结论：JZKFY 对LPS 诱导的急性肺损伤具一定的改善作用，其机制可能与改善肺血管通透性，减轻肺内中性粒细胞聚集，提高抗氧化应激能力及下调炎症反应有关。

This study was aimed to explore effects of Jinzhen Koufuye (JZKFY) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rats. Rats were randomly divided into the normal group, model group, positive control group, and three JZKFY groups with the drug dosage of 4.4, 2.2, 1.1 g·kg-1, respectively. Intragastric administration was given to animals for 7 consecutive days. One hour after the last administration, ALI model was induced by the injection of LPS (6 mg·kg-1), and then rats were killed at 16 h after saline (control) or LPS injection. Histological examinations were performed on the lungs. At the same time, the lung permeability index, activities of MPO, MDA and SOD in lung tissues, contents of TNF-ɑ, IL-6 and IL-1β in serum were measured, respectively. The results showed that JZKFY of high-, medium-, or low-dosage can significantly reduce JZKFY lung permeability index.JZKFY of high- and medium-dosage can obviously regulate activities of MPO, MDA and SOD in lung tissues, reduce contents of TNF-ɑ, IL-6 and IL-1β in serum, as well as significantly improve lung tissue lesions in ALI. It was concluded that JZKFY might improve ALI induced by LPS through inhibiting lung vascular permeability, reducing the lung neutrophil aggregation, improving the ability of antioxidative stress and lowering inflammatory response.

科学技术部国家重大新药创制项目（2013ZX090402203）：现代中药创新集群与数字制药技术平台，负责人：王振中。