目的：研究气滞胃痛颗粒组方中促胃肠动力有效组分协同关系及作用机制。方法：将大鼠随机分成16 组，分别灌胃给予不同配伍组合促胃肠动力有效组分，应用传统半固体糊碳末推进法观察大鼠用药后的胃肠动力变化；测定胃肠组织中NO、cGMP 及Ca2+含量变化。结果：气滞胃痛颗粒中促胃肠动力有效组分以枳壳黄酮和香附黄酮作用显著（P<0.01），香附挥发油和柠檬烯作用明显（P<0.05），两两交互作用表明枳壳黄酮与柠檬烯合用对胃肠动力具有显著促进作用，其次分别为枳壳黄酮与香附挥发油、香附黄酮与挥发油、柠檬烯与香附挥发油合用；各有效组分均能使胃肠组织中NO、cGMP 含量降低，Ca2+含量升高。结论：本研究确定了气滞胃痛颗粒各组分与促胃肠动力活性的相关程度及相互间协同作用，其作用机制与胃肠组织中NO、cGMP 含量降低，Ca2+含量升高有关，为进一步研究促胃肠动力药效质量控制、配伍及谱效关系提供理论依据。

This article was aimed to study the synergy effect of effective substances group and mechanisms of Qi-Zhi Wei-Tong (QZWT) Granules in promoting gastrointestinal dynamic effect in order to explore its mechanism. Rats were divided into 16 groups. Different component compatibility was given to promote the gastrointestinal dynamic effect. The traditional semi-solid paste carbon propelling analysis method was used to observe gastrointestinal motility changes of rats after medication. After intragastric administration, changes of NO, cGMP and Ca2+ content in gastrointestinal tissues were observed. The results showed that fructus aurantii flavonoids and Cyperi flavonoids had the most prominent effect in promoting gastrointestinal motility in QZWT Granules (P < 0.01), which were followed by Cyperus oil and limonene (P < 0.05). Two-way interactions indicated that the combination of fructus aurantii flavonoids and limonene had prominently promoting action in gastrointestinal motility, which was followed by the combination of fructus aurantii flavonoids and Cyperus oil, Cyperi flavonoids and Cyperus oil, limonene and Cyperus oil. Each effective component can reduce the NO and cGMP content in gastrointestinal tissues, and increase the Ca2+ content. It was concluded that the study defined the correlation and synergy between effective components and promoting effect of gastrointestinal motility. Mechanism of the effective component to promote gastrointestinal dynamic might be related to the reducing of NO and cGMP content in gastrointestinal tissues and increasing of Ca2+ content. This study also provided a theoretical basis for further research on quality control, compatibility and spectrum-effect correlation of gastrointestinal motility promotion medications.

国家自然科学基金委主任基金项目（81241111）：基于系统生物学对气滞胃痛颗粒药效组分析及作用机制研究，负责人：孟宪生。