目的：建立土知母药材中射干苷定性与定量分析方法。方法：采用TLC 法对土知母药材中射干苷进行定性鉴别；HPLC 法对土知母药材中射干苷进行含量测定。色谱柱：Diamonsil C18 （200 mm x4.6mm，5 μm），流动相：乙睛-0.2%磷酸溶液（20:80），流速：1.0 mL·min-1，波长：265 nm，柱温：25℃。结果：土知母药材定性方法专属性强，无其它成分干扰；射干苷在0.12~2.22 μg 范围内与峰面积响应值呈良好的线性关系，平均加样回收率为100.96%。结论：本法简便可行，重复性好，可用于土知母药材的质量控制。

This study was aimed to establish the quality and quantity analysis methods of tectoridin contented in the Iris tectorum Maxim. Thin layer chromatography (TLC) method was used to give quality analysis on tectoridin in I.tectorum. And the HPLC method was used to give quantity analysis on tectoridin in I. tectorum. The Diamonsil C18(200 mm x4.6 mm, 5 μm) column was used as analytical column. The acetonitrile-0.2% phosphoric acid (20:80)was used as mobile phase at the flow rate of 1.0 mL·min-1. The detection wavelength was 265 nm and the column temperature was 25℃. The results showed that the quality analysis of I. Tectorum had specific identification without the interference of other ingredients. The amount of inlet tectoridin had a good linearity with the response value of peak area in the range of 0.12~2.22 μg. The average recovery rate was 100.96%. It was concluded that this method was simple and reproducible, which can be used in the quality control of I. tectorum.

贵州省科学技术厅中药现代化科技产业研究开发专项项目（黔科合中药字[2010]5028 号）：酢酱单、艾纳香、土知母等贵州民族药材指纹图谱及质量标准提高研究，负责人：冯华。