目的：本文建立了HPLC 同时测定藏药川西千里光中绿原酸、金丝桃苷和木犀草苷3 种成分的方法。方法：采用SinoChrom ODS-BP 色谱柱（250 mmx4.6 mm，5μm），以乙腈（A）-0.1%磷酸溶液（B）为流动相，梯度洗脱，流速1 mL·min-1，检测波长355 nm，进样量10 μL，柱温30℃。结果：绿原酸、金丝桃苷、木犀草苷在30 min 内均达到基线分离，各成分在其线性范围内均呈现良好的线性关系，线性范围分别为：0.697 2~5.229 0 μg（r =0.999 4）、0.085 8~0.643 8 μg（r =1.000 0）、0.082 7~0.620 4 μg（r=1.000 0）。平均加样回收率分别为：102.09%（RSD=0.99%） 、101.17%（RSD=0.69%）、 100.91%（RSD=1.01%）。结论：本方法操作简便、准确，重复性和稳定性好，可用于川西千里光的质量控制。

This study was aimed to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of chlorogenic acid, hyperoside and luteoloside in Senecio solidagineus. Samples were analyzed on SinoChrom ODS-BP (250 mm x4.6 mm, 5 μm) with acetonitrile (A) and water containing 0.1% phosphate (B) as mobile phases for gradient elution at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 355 nm. The injection volume was 10 μL. The column temperature was 30℃. The results showed that chlorogenic acid, hyperoside and luteoloside achieved baseline separation within 30 min. A good linearity was observed in the range of 0.697 2~5.229 0 μg (r = 0.999 4), 0.0858~0.6438 μg (r = 1.000 0), 0.082 7~0.620 4 μg (r = 1.000 0) for chlorogenic acid, hyperoside and luteoloside, respectively, with the average recoveries of 102.09% (RSD = 0.99% ),101.17% (RSD = 0.69%), and 100.91% (RSD = 1.01%). It was concluded that the method was simple, accurate and reproducible, which can be used for the quality control of S. solidagineus.

国家药典委员会国家药品标准提高项目（726）：藏药双花千里光的质量标准研究，负责人：阿萍；四川省教育厅省属高校科研创新团队建设计划（11TD004）：中藏药资源保护与利用，负责人：张艺。