目的：建立同时快速测定藏药翼首草药材中绿原酸、马钱苷、獐牙菜苷、吴茱萸苷、大花双参苷A 含量的方法。方法：采用超快速液相色谱仪、Agilent Poroshell 120 SB-C18 色谱柱（100 mmx4.6 mm，2.7 μm），柱温30℃，流速1.0 mL·min-1，进样量4 μL，流动相为：乙腈-0.2%磷酸水溶液，梯度洗脱，检测波长237 nm、325 nm。结果：绿原酸、马钱苷、獐牙菜苷、吴茱萸苷、大花双参苷A 分别在8.72~218.0、1.52~38.0、2.44~61.0、29.36~734.0、3.00~75.0 μg·mL-1 范围内（r＞0.999 6，n = 6）呈良好线性关系，平均加样回收率分别为99.46% 、99.41% 、100.14% 、98.89% 、99.42% ，RSD 分别为0.69% 、0.66% 、0.60% 、1.21%、0.64%（n=9）。结论：该方法简便、准确、重复性好，可同时快速测定藏药翼首草中5 种化学成分的含量。

This study was aimed to establish an Ultra Fast Liquid Chromatography-Photo Diode Array (UFLC-PDA)method for the simultaneous determination of five chemical components, which included chlorogenic acid, loganin,sweroside, evodia rutaecarpa glycosides and triplostoside A, in Pterocephalus hookeri h eck. Agilent Poroshell 120 SB-C18 (100 mm x4.6 mm, 2.7 μm) was adopted, with acetonitrile-0.2% phosphoric acid solution in gradient elution as the mobile phase at the flow rate of 1.0 mL·min-1. And the injection volume was 0.4 μL. The detection wavelength was set up at 237 nm and 325 nm. And the column temperature was 30℃. The results showed that the calibration curve was linear within the range of 8.72~218.0, 1.52~38.0, 2.44~61.0, 29.36~734.0, 3.00~75.0 μg·mL-1 (r > 0.999 6, n =9) for chlorogenic acid, loganin, sweroside, evodia rutaecarpa glycosides and triplostoside A, respectively. The average recovery rates were 99.46%, 99.41%, 100.14%, 98.89%, and 99.42%, respectively. The RSD was 0.69%, 0.66%, 0.60%, 1.21%, and 0.64%, respectively (n = 9). It was concluded that this method was simple, accurate and reproducible, which can be used for the simultaneous determination of the content of five chemical components in P. hookeri.

国家自然科学基金委面上项目（81274193）：基于肠道菌群代谢-体内吸收-药效相关性的藏药翼首草治疗“真布病”药效物质基础研究，负责人：张艺。