目的：阐明彝药“麻补”（狭叶重楼）的止血活性物质基础及机理。方法：采用植物化学技术从“麻补”药材中分离鉴定出一种C27甾体皂苷类化合物——重楼皂苷H（PSH）。测定了PSH 对正常小鼠的断尾出血时间（BT）、凝血酶原时间（PT）、活化的部分凝血活酶时间（APTT）、血浆纤维蛋白原（FIB）等凝血功能指标的影响。结果：PSH 可显著缩短BT，显示出明显的止血活性；PSH 对缩短正常小鼠PT、APTT 作用不明显，但可显著提高FIB。结论：重楼皂苷H 对内源凝血通路及外源凝血通路无明显促进作用，PSH 升高FIB 为其发挥止血活性的重要途径。

This study was aimed to carry out pharmacological research on the hemostatic activity and mechanism of Yi medicine "Ma-Bu" (Paris polyphylla Smith var stenophylla Franch.). One kind of C27 steroidal saponin from P.polyphylla Smith var stenophylla Franch. was isolated and identified as Paris saponin H (PSH). The effect of PSH on the index of bleeding time (BT), prothrombin time (PT), activated partial thromboplastin time (APTT) and fibrinogen (FIB) of mice were measured. The results showed that PSH have significant hemostatic activity by shortening BT. The effect of PSH on shortening PT and APTT of mouse was not significant. However, the FIB was enhanced significantly after treatment with PSH. It was concluded that PSH had no significant promoting effect on the extrinsic coagulation pathway (ECP) or the intrinsic coagulation pathway (ICP). The enhancement of FIB may be a pathway for the effect of hemostatic activity by PSH.

霍英东教育基金会教育基金（121045）：基于C27 甾体皂苷分子进化的中药“蚤休”基源的化学分类学研究，负责人：尹鸿翔；国家自然科学基金委青年项目（81001606）：“同属多基源”蚤休类生药的植物化学分类学特征及品质评价研究，负责人：尹鸿翔；四川省教育厅自然科学面上项目（11ZB049）：“同属多基源”中药重楼的化学分型及质量评价，负责人：尹鸿翔；成都中医药大学科技发展基金（ZRMS201245）：彝药“麻补”止血活性物质基础及品质评价研究，负责人：尹鸿翔；四川省教育厅创新团队支持项目（11TD004）：特色中药资源的创新研究与开发团队，负责人：张艺。