目的：研究雄芍汤抗肝纤维化的药效物质基础，分别筛选其保肝降酶及抗氧化的有效部位。方法：Wistar 雄性大鼠随机分为正常组、模型组、扶正组、雄芍组、多糖组、总碱组和总苷组，采用DMN 腹腔注射法制备大鼠肝纤维化模型，造模成功后灌胃给药，治疗组分别给予扶正化瘀胶囊（0.105 g·mL-1）、雄芍汤药液（1.610 g·mL-1）、雄芍汤粗多糖提取物（35.420 mg·mL-1）、雄芍汤总苷提取物（25.725 mg·mL-1）、雄芍汤总生物碱提取物（0.196 mg·mL-1），正常组与模型组灌服等量生理盐水，每日1 次，疗程4 周。4 周后处死取材，全自动生化分析仪检测血清肝功能指标ALT、AST、TIBL 和ALB；黄嘌呤氧化酶法检测血清SOD活力；DTNB 还原法检测血清GSH-PX 活力；TBA 法检测血清MDA 含量；HE 染色及Masson 染色观察肝组织病理学改变。结果：与模型组比较，除总碱组无显著性差异外，其余各治疗组ALT、AST 及TBIL 均显著降低（P<0.05 或P<0.01），ALB 均显著升高（P<0.05 或P<0.01）。与模型组比较，扶正组、雄芍组和总碱组SOD 及GSH-PX 均显著升高（P<0.01），MDA 均显著降低（P<0.05 或P<0.01），多糖组、总苷组无显著性意义。结论：粗多糖部位和总苷部位为雄芍汤保肝降酶的有效部位，总生物碱部位为雄芍汤抗氧化的有效部位。

This article was aimed to study the therapeutic material basis of Xiong-Shao (XS) decoction on hepatic fibrosis (HF), and screen effective parts from XS decoction for protecting liver, reducing enzyme activity and oxidative damage. Male wistar rats were randomly divided into the normal group, model group, Fu -Zheng Hua-Yu (FZHY) group, XS group, polysaccharide group, total alkaloids group and the total glycosides group. HF rat model was established with the intraperitoneal injection of DMN. After modeling, FZHY solution (0.105 g·mL-1), XS decoction (1.610 g·mL-1), crude polysaccharides extract of XS decoction (35.420 mg·mL-1), total glycosides extract solution (25.725mg·mL-1), and total alkaloids extract of XS decoction (0.196 mg·mL-1) were administered to corresponding treatment group by gavage once a day for 4 weeks, respectively. Rats of the normal group and model group were given equivalent normal saline by gavage once a day for 4 weeks. After 4-week drug administration, rats were killed to remove the liver. Automatic biochemical analyzer was used in the detection of serum parameters of liver function, including ALT, AST, TIBL and ALB. Serum SOD activity was detected by xanthine oxidase. GSH-PX activity was tested by DTNB reduction. Serum contents of MDA were measured by TBA. Pathological changes of the liver were observed with HE staining and masson staining. The results showed that compared with the model group, there was no significant differences between the total alkaloids group and the model group, but levels of serum ALT, AST and TBIL of other treatment groups were significantly decreased, and the serum ALB level was significantly elevated (P < 0.05 or P < 0.01). Compared with the model group, levels of serum SOD and GSH-PX of the FZHY group, XS group and total alkaloids group were significantly elevated (P < 0.01), and level of serum MDA was significantly reduced (P <0.05 or P < 0.01). Comparison among the polysaccharides group, total glycosides group, and model group showed no significant differences. It was concluded that crude polysaccharide and total glycosides fractions were effective parts of XS decoction for protecting liver and reducing enzyme activity. And total alkaloids fraction was the effective part for reducing oxidative damage.

山西省科学技术厅青年科技研究基金项目（2012021031-4）：雄芍汤有效部位抗肝纤维化的作用及机理实验研究，负责人：李霞。