目的：研究内生生防细菌在人参根、茎、叶和根区土壤中的消长动态。方法：采用浓度梯度筛选法获得抗利福平标记菌株，灌根法接种标记菌株，稀释平板法计数标记菌株在人参根、茎、叶和根区土壤的分布情况。结果：通过抗生素诱导培养，最终获得了能够耐受120 μg·mL-1 利福平的标记菌株，其对人参黑斑病菌和人参疫病菌的抑制作用与诱导前未见明显改变。内生菌株ge15 和ge25 在人参根、茎中的初始菌落数最高，随后ge15 和ge25 在人参根、茎中的菌落数逐渐平稳并维持特定浓度；在人参叶中，两株菌在接种3 天后菌落数达到最高，后逐渐降低直至趋于平稳；在人参根区土壤，ge15 的菌落数往复变化，ge25 的菌落数先上升后下降，最后菌落数趋于平稳。结论：两株内生菌均能够在人参根茎叶和根区土壤中维持特定菌群浓度，基于上述菌株的生防制剂将有助于人参病害的田间防治。

This article was aimed to study the dynamics of biocontrol endobacteria in roots, stems and leaves of Panax ginseng and soils adjacent roots. Gradient screening and root irrigation methods were used to develop Rifresistance bacteria and inoculation. Dilution plate method was used to count the number of them in roots, stems and leaves of P. ginseng and soils adjacent roots. The results showed that bacteria which can endure 120 μg·mL-1 Rif was developed, and no change was found on their antagonistic activity against Alternaria panax Whetz and Phytophthora cactorum. In roots and stems of P. ginseng, ge15 and ge25 showed the highest number in the initial.Then, populations of ge15 and ge25 in ginseng roots and stems decreased to a stable status gradually. In ginseng leaves, populations of ge15 and ge25 increased to the highest 3 days after inoculation, then, populations of them were decreased and kept at certain concentration. In ginseng rhizosphere soil, populations of ge15 showed reciprocating change, and which of ge25 showed increase initial, and then decrease to a stable status. It was concluded that endobacteria can keep a certain population in ginseng roots, stems, and leaves. Biocontrol agents based on them will be helpful for ginseng diseases controlling in the field.

国家中医药管理局中央公益型科研院所基金项目（2012D13）：人参内生细菌ge25 菌株的应用评价研究，负责人：李勇；中国医学科学院协和青年科学基金项目（332013113）：人参内生菌株ge25 可用性评价及菌剂制备工艺研究，负责人：李勇。