[关键词]
[摘要]
目的:对云南文山三七根际土壤中酚酸的种类进行鉴定,并对其进行含量测定。方法:采用HPLC-MS、HPLC 技术,并通过文献和对照品比对,鉴定了云南文山三七根际土壤中酚酸的种类并对其进行含量测定。结果:从三七根际土壤提取液中检测到对羟基苯甲酸、香草酸、丁香酸、对香豆酸、阿魏酸和苯甲酸6 种酚酸类物质,其中对香豆酸含量最高(24.01 μg·g-1),丁香酸含量最低(1.26 μg·g-1)。6 种酚酸类成分在三七根际土壤中的含量顺序为对香豆酸>香草酸>苯甲酸>对羟基苯甲酸>阿魏酸>丁香酸。结论:三七根际土壤中的酚酸类物质可能为三七植物须根残体腐解产生,也可能是三七根系分泌产生。
[Key word]
[Abstract]
Phenolic acids of rhizosphere soil of Panax notoginseng were identified and the contents were determined. HPLC-MS and HPLC were used. Compared with the literature and standard reference, identification and content determination were given on phenolic acids of rhizosphere soil of Panax notoginseng in Wenshan, Yunnan province. The results showed that six types of phenolic acids were detected from the extract of rhizosphere soil of Panax notoginseng, which were p-hydroxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid, ferulic acid and benzoic acid.The content of p-coumaric acid showed the highest concentration of 24.01 μg·g-1, while syringic acid had the lowest concentration of 1.26 μg·g-1. The sequence of content in the rhizosphere soil of Panax notoginseng from top to bottom was p-coumaric acid, vanillic acid, benzoic acid, p-hydroxybenzoic acid, ferulic acid and syringic acid. It was concluded that the rhizosphere soil of Panax notoginseng contained six types of phenolic acids, which were p-hydroxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid, ferulic acid and benzoic acid.
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[基金项目]
国家自然科学基金委青年项目(81102751):营养胁迫下三七化感作用变化及其与连作障碍机理间的关系研究,负责人:张子龙;北京中医药大学研究生自主课题(2013-JYBZZ-XS-070):连作三七土壤中皂苷的动态含量测定以及皂苷对三七的化感自毒作用研究,负责人:吴立洁。