目的：建立补骨脂药材的超高效液相色谱（UHPLC）指纹图谱。方法：以Shim-pack XR-ODSⅢ（50 mmx2.0 mm，1.6 μm）为色谱柱，乙腈-0.2%冰醋酸溶液为流动相，梯度洗脱，流速为0.1 mL·min-1，检测波长为246 nm，柱温45℃。结果：建立了补骨脂药材的UHPLC 指纹图谱，标定10 个共有色谱峰，并通过对照品比对指认了其中6 个色谱峰。结论：该方法快速、可靠、重复性好，可为补骨脂药材的物质基础和质量控制方法的研究提供参考。

This study was aimed to establish the ultra high performance liquid chromatography (UHPLC) fingerprints of Psoralea corylifolia L. The separation was achieved on a Shim-pack XR-ODS Ⅲcolumn (50 mm x2.0 mm, 1.6 μm) by gradient elution with acetonitrile-0.2% glacial acetic acid solution as the mobile phase. The flow rate was 0.1 mL·min-1 and the measurement wavelength was 246 nm. The temperature of the column was 45℃. The results showed that the UHPLC fingerprint of P. corylifolia L. was established and 10 characteristic common peaks were found, among which 6 peaks were recognized by comparing with reference substances. It was concluded that the method was rapid, reliable and reproducible. The established fingerprint can provide references for the study of substance basis and quality control of P. corylifolia L.

重庆市卫生局中医药科技项目（ZY20132068）：一测多评法优化盐补骨脂的炮制工艺及质量评价，负责人：励娜。