目的：运用LC-MS/MS 技术对斑蝥中斑蝥素类化合物进行成分分析鉴定。方法：待分析组分经HPLC 选择合适的分离条件后进行LC/MS/MS 分析，经正、负离子预扫描，选择正离子扫描方式对各个分离成分进行扫描，得到扫描图谱，推断相对分子质量。结果：共分析鉴定8 个斑蝥素类化合物，其中3 个为同分异构体。结论：LC-MS/MS 能快速、准确地鉴定斑蝥中斑蝥素类化学成分，以结合氨基酸的形式存在体内。

This study was aimed to verify the chemical component cantharidin from Chinese blister beetle by LCMS/MS. Components were first processed by HPLC to choose the appropriate separation conditions before LC/MS/MS analysis. Through the positive and negative ions pre-scan, positive ions scanning way was selected in the scanning of each separate component. The scanned maps were obtained and the relative molecular mass was deduced. The results showed that 8 types of cantharidin compounds were identified, including three isomers. It was concluded that LCMS/MS was able to determine the chemical component cantharidin from Chinese blister beetle rapidly and accurately,which was existed in the body by the form of combined amino acid.

重庆市科委科研院所创新能力建设项目（cstc2012pt-kyys10004）：中药新产品开发研究能力提升建设，负责人：唐安明；重庆市科委科研院所创新能力建设项目（cstc2012pt-kyys10001）：重庆市GLP 中心能力提升建设，负责人：杨大坚。