目的：HPLC 法同时测定运脾散浸膏中柚皮苷、橙皮苷和苍术素的含量。方法：色谱柱：Thermo ODS-2（250 mmx4.6 mm，5 滋m）；流动相：甲醇-水梯度洗脱，0~5 min（30%~40% A），5~15 min（40%~50%A），15~20 min（50%~79% A），20~30 min（79%~100% A），30~45 min（100% A），45~55 min（100%~30%A），55~60 min（30% A）；柱温：30℃；检测波长：283 nm；流速：1 mL·min-1。结果：柚皮苷、橙皮苷、苍术素线性范围分别为0.009 16~0.137 00 μg（r=0.999 9）、0.006 22~0.091 30 μg（r=0.999 8）、0.003 58~0.053 70 μg（r=0.999 9），平均回收率分别为103.51%、96.82%、97.65%，RSD 分别为1.37%、1.41%、1.49%（n=6）。结论：本方法专属性强，重复性好，可用于运脾散浸膏中柚皮苷、橙皮苷和苍术素的含量测定。

This study was aimed to simultaneously determinate the content of naringin, hesperidin and atractylodin in Yun-Pi (YP) Powder extract by HPLC. A Thermo ODS-2 column (250 mm x4.6 mm, 5 μm) was used for the gradient elution with a methanol-water of 0~5 min (30%~40% A), 5~15 min (40%~50% A), 15~20 min (50%~79%A), 20~30 min (79%~100% A), 30~45 min (100% A), 45~55 min (100%~30% A), 55~60 min (30% A). The column temperature was at 30℃. The detection wavelength was at 283 nm. The flow rate was 1 mL·min-1. The linear range was determined to be 0.009 16~0.137 00 μg (r = 0.999 9), 0.006 22~0.091 30 μg (r = 0.999 8), 0.003 58~0.053 70 μg (r = 0.999 9), respectively for naringin, hesperidin and atractylodin. The average recovery rate of pterodontic acid was 103.51%, 96.82% and 97.65%. And RSD was 1.37%, 1.41%, 1.49% (n = 6). It was concluded that the method had good specificity and repeatability. It was considered as a good tool for the content determination of naringin, hesperidin and atractylodin in YP Powder extract.

贵阳市科技局科技计划项目[筑科合同（2012204）46 号]：运脾颗粒的研制，负责人：刘文；国家中医药管理局2010 年黄建业名老中医药专家传承工作室建设项目[中国中医药人教发（2010）59 号]：黄建业名老中医药专家传承工作室建设，负责人：彭玉。