目的：建立HPLC 法同时测定尖叶假龙胆中的1，5-dihydroxy-3-methoxyxanthone 8-O-β-Dglucopyranoside（F1）、1-hydroxy-3,4-dimethoxyxanthon 7-O-β-D-glucopyranoside （F2）、1,8-dihydroxy-3,4-dimethoxyxanthone 5-O-β-D-glucopyranoside（F3）3 个[口山]酮苷的含量。方法：Thermo syncronis C18（4.6 mm× 250 mm，5 μm）色谱柱，乙腈-水（24.5∶75.5，V/V）等度洗脱，流速1.0 mL·min-1，检测波长为254 nm，柱温为35益。结果：F1、F2、F3 分别在14.06~281.28 μg·mL-1（R2=0.999 7）、0.56~11.16 μg·mL-1（R2=0.999 8）、0.46~9.20 μg·mL-1（R2=0.999 9）范围内线性关系良好；平均回收率（n=9）分别为99.70%（RSD=1.06%）、99.78%（RSD=1.21%）、100.28%（RSD=1.15%）。结论：该方法能快速、简便地测定尖叶假龙胆中3 个主要的[口山]酮苷的含量，为该药材的质量控制研究提供了参考。

To develop a method for determining 3 Xanthone glycosides (1, 5-dihydroxy-3-methoxyxanthone 8-O-β-D-glucopyranoside(F1), 1-hydroxy-3, 4-dimethoxyxanthon 7-O-β-D-glucopyranoside (F2), 1, 8-dihydroxy-3, 4-dimethoxyxanthone 5-O-β-D-glucopyranoside (F3) of Gentianella acuta by HPLC. The Thermo syncronis C18 (4.6mm×250 mm, 5 μm) was used for simultaneous determination of 3 Xanthone glycosides in G. acuta. Isocratic elution with water and acetonitrile was 75.5∶24.5. The flow rate was 1.0 mL?min-1 and the detection wavelength was set at 254 nm. The column temperature was 35℃. The linear concentration ranges of F1, F2, F3 were 14.06 ~ 281.28 μg?mL-1 (R2=0.999 7), 0.56~11.16 μg?mL-1 (R2=0.999 8), 0.46~9.20 μg?mL-1 (R2=0.999 9), respectively; The average recoveries (n = 9) were 99.70% (RSD=1.06%), 99.78% (RSD=1.21%), 100.28% (RSD=1.15%), respectively. The method is simple, accurate and sensitive, and can be used for the quality control of G. acuta as a reference.

科学技术部国际科技合作项目（2010DFB33260）：中医药干预治胰岛素抵抗创新中药研究，负责人：徐暾海；北京中医药大学创新团队项目（2011-CXTD-19）：中医药干预糖尿病及其并发症研究团队，负责人：刘铜华。