目的：观察4种补肾中药淫羊藿、补骨脂、女贞子、何首乌有效成分淫羊藿苷、补骨脂素、齐墩果酸、二苯乙烯苷正交配伍，两种非补肾中药川芎、黄芪有效成分川芎嗪、黄芪甲苷，对大鼠骨髓间充质干细胞（BMSCs）的调控作用。方法：65 只SD 大鼠随机分为正常对照组、阳性转化液对照组、补肾配伍组（1组、2组、3组、4组、5组、6组、7组、8组、9组）、非补肾药对照组（黄芪甲苷组、川芎嗪组）。补肾配伍组、非补肾药对照组予灌胃给药，每日1次，连续3天。正常对照组、阳性转化液对照组予等剂量生理盐水灌胃。灌胃第3天，大鼠处死后取各组含药血清培养骨髓间充质干细胞6、12、18天；ELISA法分别定量检测6、12、18天骨形态发生蛋白2（Bmp2）活性表达及含量，评价成骨细胞分化程度。用实时定量PCR法检测含药血清培养骨髓间充质干细胞第18 天Bmp2、Smad1、Smad4 mRNA表达。结果：补肾配伍药可提高体外培养骨髓间充质干细胞Bmp2的活性表达及含量，在12天达到高峰值。补肾配伍组能上调Bmp2、Smad1、Smad4的mRNA表达。结论：补肾中药有效成分配伍能促进骨髓间充质干细胞分化为成骨细胞,其机制可能与上调Bmp2、Smad1、Smad4 mRNA表达量及Bmp2的活性及含量有关。

This study was aimed to observe four kinds of kidney-tonification medicine, which were Epimedium, psoralen, Ligustrum lucidum, Polygonum with the active ingredient of icariin, psoralen, oleanolic acid, stilbene glucoside and their orthogonal compatibility. There were two kinds of non-kidney tonification medicine, which were Chuanxiong and astragalus with the active ingredient of TMP and astragaloside. The observation was made on the regulatory role of rat bone marrow stem cells (BMSCs). A total of 65 SD rats were randomly divided into the normal control group, positive transformed control group, kidney-tonification compatibility group (including Group 1, Group 2, Group 3, Group 4, Group 5, Group 6, Group 7, Group 8, and Group 9), non-kidney tonification medicine control group (including TMP group and astragaloside group). Intragastric administration of medication was given to the kidney-tonification compatibility group and the non-kidney tonification medicine control group, once a day for 3 consecutive days. Intragastric administration of equal amount of normal saline was given to the normal control group and the positive transformed control group. On the third day of intragastric administration, rats in each group were sacrificed. Serum containing medication was used in the culture of BMSCs for 6, 12, or 18 days. ELISA method was used to quantitatively detect the expression activity and content of BMP2 on the 6th, 12th, or 18th day, in order to evaluate the degree of bone cell differentiation degree. Real-time quantitative PCR method was used for detection of expression of Bmp2, Smad1, Smad4 mRNA in serum containing medication in the culture of BMSCs on the 18th day. The results showed that the kidney-tonification compatibility can improve the expression activity and content of BMP2 culture in vitro, with the peak on the 12th day. The kidney-tonification compatibility groups can upregulate expressions of Bmp2, Smad1, Smad4 mRNA. It was concluded that the active ingredient compatibility of kidney -tonification medicine can promote BMSCs. Its mechanism may be related to the upregulation of expression of Bmp2, Smad1, Smad4 mRNA, and the activity and content of Bmp2.

科学技术部国家重点基础研究发展（“973”）计划（2010CB53040-04）：基于“肾藏精”的脏象理论研究-从骨质疏松症探讨“肾主骨”理论的研究，总课题负责人：王拥军，子课题负责人：郑洪新。