目的:观察补气活血类中药含药血清对体外培养的人脐静脉血管内皮细胞（HUVEC）迁移的影响，并初步筛选出对HUVEC 迁移能力具有较明显的促进、抑制作用的药物。方法：建立HUVEC的体外培养的细胞模型，将赤芍、川芎、丹皮、丹参、莪术、三棱、黄芪、人参按照1∶2，1∶1，2∶1 3种配比两两配伍组合，组成13对补气活血药药对，采用血清药理学方法，制备含药血清，应用划痕法观察不同活血化瘀药和补气活血药药对的10%含药血清对HUVEC（5×105/mL 密度）作用24 h后迁移能力的影响。结果：与空白血清组相比较，丹参组、川芎组、赤芍组，丹参黄芪（1∶2）组、丹参黄芪（1∶1）组、川芎黄芪（1∶2）组对血管内皮细胞的迁移能力具有明显促进作用（P＜0.05或P＜0.01）；三棱组、莪术组、丹皮组，莪术黄芪（2∶1）组、三棱人参（2∶1）组、莪术黄芪（1∶1）组对血管内皮细胞的迁移能力具有明显抑制作用（P＜0.05或P＜0.01）。结论：不同的活血化瘀药和补气活血药配伍组成对HUVEC 的迁移能力具有不同的促进或抑制作用，这可能是这些药物在细胞层面上促进或抑制血管生成的作用机制之一。

This study was aimed to observe the influence on migration ability of human umbilical vein endothelial cell (HUVEC) in vitro by couplet medicines of reinforcing qi and activating blood, and initially screen medicines with relative obvious promoting or inhibiting effect on migration ability of HUVEC. The model of HUVEC cultured in vitro was established. The Paeonia, Ligusticum chuanxiong, Cortex moutan, Salvia, curcuma zedoaria, Sparganium, Astragalus, and ginseng were combined in pairs with the proportion of 1∶2, 1∶1, 2∶1. There were 13 couplet medicines of reinforcing qi and activating blood. Serum pharmacological method was used to prepare medicated serum of the couplet medicines of reinforcing qi and activating blood. Scratch method was used to detect the effect of medicated serum of these couplet medicines of reinforcing qi and activating blood and activate blood herbs on the migration ability of HUVEC (with the density of 5×105/mL) after 24 hours. The results showed that compared with the blood serum of the blank group, the Cortex moutan group, Ligusticum chuanxiong group, Paeonia group, Salvia and Astragalus (1∶2) group, Salvia and Astragalus (1∶1) group, Ligusticum chuanxiong and Astragalus (1∶2) group had obvious promoting effect on the migration ability of VEC(P < 0.05 or P < 0.01). The Sparganium group, curcuma zedoaria group, Cortex moutan group, curcuma zedoaria and Astragalus(2∶1) group, Sparganium and ginseng (2∶1) group, Sparganium and Astragalus (1∶1) group had obvious inhibiting effect on the migration ability of VEC (P < 0.05 or P <0.01). It was concluded that different compatibility of medicines of reinforcing qi and activating blood and activate blood herbs had different promoting or inhibiting effect on migration ability of HUVEC. It may be related to the mechanism of action of these drugs on promoting or inhibiting angiogenesis at the cellular level.

国家自然科学基金委面上项目（81073072）：基于活体成像技术的补气活血药配伍抗卵巢癌转移时效关系研究，负责人：唐德才；国家自然科学基金委面上项目（81373990）：黄芪、莪术配伍调控肝癌血管生成的体视学及分子机制研究，负责人：唐德才；江苏省人力资源和社会保障厅六大人才高峰项目（013034010004）：基于SDF-1/CXCR4 通路的补气活血药配伍对血管生成的调控规律研究，负责人：唐德才。