目的：通过制备苗药组方雾化剂，观察苗药组方雾化剂对染矽尘大鼠血清SOD和MDA及肺组织细胞凋亡Fas 和Caspase-3基因表达的影响。方法：健康SD大鼠分成5组，其中4组进行染矽尘造模，待模型成功后随机选其中3 组分别给予糖皮质激素雾化、苗药组方雾化剂雾化、苗药组方雾化加中药灌胃治疗，治疗40天后，采用WST-1法检测超氧化物歧化酶（SOD），TBA法检测丙二醛（MDA），提取实验大鼠肺脏组织RNA，采用反转录合成cDNA，通过RT-PCR技术检测Fas、Caspase-3基因表达。结果：与染矽尘组比较，苗药组方雾化组大鼠血清中SOD 活力明显升高（P＜0.05），苗药组方雾化组大鼠血清中MDA 含量明显降低（P＜0.05），苗药组方雾化组大鼠肺组织Fas和Caspase-3基因表达明显降低（P＜0.05）。结论：苗药组方雾化组能增强染矽尘大鼠血清SOD活力，减少染矽尘大鼠血清MDA含量，显著降低大鼠肺组织Fas和Caspase-3基因表达。

This study was aimed to prepare the spraying agent of prescriptions of Miao nationality herb and investigate the effect of Miao nationality herbs spray for serum SOD, MDA, and expression of Fas and Caspase-3 mRNA in lung tissues of silica-treated rats. The healthy SD rats were divided into 5 groups. Silica dust suspension was used in the model establishment of 4 groups. After the model was successfully established, 3 groups were randomly selected and given glucocorticoids atomization inhalation, Miao nationality herbs spray, Miao nationality herbs spray combined with intragastric administration of herbal medicine, respectively. After 40 -day treatment, water-solubletetrazolium salt (WST-1) was used in the detection of serum superoxide dismutase (SOD). Thiobarbituric acid (TBA) was used in the detection of malondialdehyde (MDA). The mRNA expression variance of the Fas and Caspase-3 were detected by RT-PCR. The results showed that compared with the silica dust suspension group, the SOD activity of serum in the Miao nationality herbs spray group was significantly increased(P < 0.05). MDA content and the mRNA of Fas and Caspase-3 were significantly lower in the Miao nationality herbs spray group(P < 0.05). It was concluded that Miao nationality herbs spray group was able to increase the SOD activity of serum, decrease MDA content, and obviously decrease the expression of Fas and Caspase -3 of lung tissues among silica dust suspension rats.

贵阳市科技局科技计划项目（筑科合同[2011103]28 号）：苗药组方雾化剂的制备及其对矽肺治疗作用的研究，负责人：黄高。