目的：本实验主要研究650 nm半导体激光不同能量密度照射足三里穴对急性佐剂性关节炎的治疗差异，研究激光照射的生物效应及其作用。方法：健康雄性SD大鼠60只，随机分为正常组对照组、模型组、低能量密度组（61.89 J/cm2）、高能量密度组（247.57 J/cm2），将弗式完全佐剂（FCA）注入大鼠右后肢足跖皮内，建立佐剂性关节炎模型（AA），治疗组采用650 nm半导体激光照射，分别用61.89 J/cm2、247.57 J/cm2两种能量密度照射大鼠足三里穴，观测各组大鼠造模后，第1、3、5次治疗后关节肿胀度、痛阈、肿瘤坏死因子-α（TNF-α）、白介素-1β（IL-1β）水平的变化。结果：与正常组比较，模型组关节肿胀度、痛阈、TNF-α、IL-1β明显升高（P<0.05），完成5 次治疗后低能量和高能量密度的半导体激光对于急性炎症均有效果，高能量密度组治疗效果优于低能量密度组（P<0.05）。结论：61.89 J/cm2和247.57 J/cm2能量密度半导体激光照射足三里穴可以缓解急性AA 带来的炎症疼痛、肿胀，降低TNF-α、IL-1β浓度水平。半导体激光照射具有刺激或抑制、累积作用后及抛物线特征。低能量密度组获得最佳疗效位于第3~5次治疗之间。

This study was aimed to compare the difference of treatment on acute adjuvant arthritis (AA) by using different energy densities withirradiation on ST36-Zusanli under the fixed wavelength (650 nm) by the semiconductor. Biological effect and traits of the laser irradiation were also studied. A total of 60 healthy male SD rats were randomly divided into the normal control group, model group, low energy density group (61.89 J/cm2)and high energy density group (247.57 J/cm2). The Freund's complete adjuvant (FCA) was intradermallyinjected into rats' right hind paw to establish AA rat model. The treatment group was treated with 650nm semiconductor laser irradiation on ST36-Zusanli with the energy density of 61.89 J/cm2 and 247.57 J/cm2, respectively. The joint swelling degree, pain threshold, TNF-α and IL-1β level changes were observed on thefirst, third and fifthtreatment after modeling. The results showed that compared with the normal group, the joint swelling degree, pain threshold, TNF-α, and IL-1β were obviously increased in the model group (P<0.05). After five times treatment, the semiconductor laser with low and high energy density had effect on acute inflammation. And the high energy density treatment effect had a better effect than the low energy density one (P<0.05). It was concluded that the energy density of 61.89 J/cm2 and 247.57 J/cm2 of semiconductor laser irradiation on ST36-Zusanli can relieve acute inflammatory pain and swelling caused by AA; reduce the TNF-α and IL-1β concentration levels. Semiconductor laser irradiation had the characteristics of stimulation or inhibition, cumulative effect and parabola. Low energy density group obtained the best effect between the third and fifth treatment.

教育部博士点基金联合资助课题（20113107110011）：Ga-Al-As 半导体激光干预穴位光生物学效应诱发骨骼肌细胞线粒体、细胞凋亡及细胞能量畸变的机制研究，负责人：杨华元。