目的：以PT、APTT、TT、FIB、血小板聚集率及纤溶图参数为指标，评价土鳖虫抗凝组分F2-2的体内抗凝药效。方法：大鼠皮下注射肾上腺素5 天造成急性血瘀症模型后，对模型大鼠灌胃给药土鳖虫抗凝组分F2-2，9天后对各指标进行测定。结果：与模型组相比，灌胃给药后的大鼠PT/APTT均延长、FIB含量降低、血小板聚集率与血液最大凝固程度下降，TT则无差异。结论：土鳖虫抗凝组分F2-2有良好的体内抗凝药效，其作用机制尚有待进一步研究。

Prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), plasma fibrinogen (FIB), platelet aggregation rate and blood clots-fibrinolytic dynamic figure were taken as indexes in the evaluation of anticoagulant activity in vivo of active component F2-2 from Eupolyphaga seu Steleophaga. After 5 days of hypodermic injection of adrenaline, the rat model of acute blood stasis was established. Indexes were determined after the model rats were treated with an intragastric administration of F2-2 for 9 days. The results showed that compared with the model group, PT/APTT was prolonged, FIB content was decreased, platelet aggregation rate and the largest of blood coagulation were declined after 9 days of intragastric administration in the model group. However, there was no difference on TT. It was concluded that the anticoagulant component F2-2 separated from E. seu Steleophaga showed favorable anticoagulant activity in vivo. However, its mechanism remained unknown and requested further researches.

科学技术部国家“973”重点基础研究计划（2012CB518606）：通络药物物质基础、作用机制及临床评价研究，负责人：吴以岭。