目的：应用血小板生物亲和萃取-HPLC分析法分析栀子提取物中抗血小板聚集的活性成分，并对活性成分进行药理实验，以验证血小板生物亲和萃取-HPLC分析法在栀子提取物抗血小板聚集效应物质分析中的可行性。方法：将栀子提取物在生理条件下与洗涤血小板相互作用，洗去非结合成分，调节pH破坏血小板结构后洗脱结合成分，将各洗脱液用已建立的指纹图谱方法进行HPLC分析，找出准活性成分，并进一步对活性成分进行药理实验。结果：可检测到栀子提取物中的主要与血小板结合成分为栀子苷，药理实验也进一步证实一定剂量范围栀子苷对由ADP、鼠尾胶原及凝血酶诱导的大鼠血小板聚集具有明显的抑制作用（P<0.01）。结论：血小板生物亲和萃取-HPLC分析法可以用于分析栀子提取物与血小板膜受体的相互作用，所筛选的活性成分与其药理作用有一定的相关性。

This study was aimed to search anti-platelet aggregation effectors from Gardenia jasminoides extract with the employment of platelet affinity extraction method coupled with HPLC, in order to provide pharmacological experimental evidences of the selected effectors to verify its feasibility. Under physiological conditions, washed rat platelets were added into G. jasminoides extract and then a mixture was gained. Consequently, some components from G. jasminoides extract were combined to the platelets in the mixture while some were not owing to their special chemical structures and properties. Firstly, the uncombined components were washed off from the mixture. Secondly, the combined components in the leftover was washed down and collected, respectively, right after destroying the occupied platelets' structures. Thirdly, different collected eluents were analyzed, respectively, by HPLC established in the previous work to search the effectors. Fourthly, pharmacological experiments were implemented for confirmation. The results showed that dominant effective components from G. jasminoides extract acting on anti-platelet aggregation were identified as geniposide. Further evident was provided as well by pharmacological experiment that geniposide exhibited significant inhibitory effect on anti-platelet aggregation in rats induced by ADP, rat tail collagen and thrombin(P < 0.01). It was concluded that the platelet affinity extraction-HPLC method proposed in this paper can be utilized to analyze the correlation of effectors from G. jasminoides extract and its pharmacological effects. Moreover, there are some correlations between screened chemical substances and their pharmacological effects.

科学技术部国家科技重大新药创制专项（2009ZX09502-008，2009ZX09308-003）：基于“脑络痹阻、毒损脑络”病机治疗老年性痴呆的三七、栀子组分新药-SZ52（中药5类），负责人：杜守颖；科学技术部国家重点基础研究发展“973计划”（2012CB724001）：基于释药动力学的多组分缓控释给药体系的评价方法研究-栀子，负责人：杜守颖；北京中医药大学创新团队（2011-CXTD-13）：复方中药制药研究创新团队，负责人：杜守颖。