目的：探讨热毒宁注射液解热镇痛作用。方法：SD大鼠背部皮下注射酵母复制发热模型，尾静脉注射热毒宁注射液，剂量分别为10.16 g?kg-1、5.08 g?kg-1、2.54 g?kg-1，观察不同时间动物体温变化；ICR小鼠连续7日尾静脉注射热毒宁注射液，剂量分别为20.30 g?kg-1、10.15 g?kg-1、5.08 g?kg-1，采用扭体法、热板法和小鼠爪痛测试进行镇痛实验。结果：热毒宁注射液高剂量和中剂量可以明显降低发热动物的体温（P<0.05或P<0.01），同时均可明显降低醋酸所致小鼠的扭体次数（P<0.05或P<0.01），明显提高小鼠热板和爪痛的痛阈值（P<0.05或P<0.01）。结论：热毒宁注射液具有较好的解热镇痛作用。

This article was aimed to study the analgesic and antipyretic effect of Re-Du-Ning(RDN) Injection. Heating model was building on SD rats after subcutaneous injection of yeast. And then, RDN Injection was intravenous injected at the dose of 10.16 g?kg-1, 5.08 g?kg-1, and 2.54 g?kg-1, respectively. Observation was made on changes of rats' temperature. RDN Injection was intravenous injected into ICR mice at the dose of 20.30 g?kg-1, 10.15 g?kg-1, 5.08 g?kg-1 for 7 consecutive days. The methods of mice twist, hot plate and jaw pain were used in the testing of analgesic action of RDN Injection. The results showed that RDN Injection at high and middle dose can significantly reduce the temperature of heating rats models (P < 0.05 or P < 0.01). It can also significantly reduce the twisting times of mice by acetic acid (P < 0.05 or P < 0.01). It can also significantly increase the pain threshold of mice by hot plate and jaw pain (P < 0.05 or P < 0.01). It was concluded that RDN Injection had a good analgesic and antipyretic effect in mice.

科学技术部重大新药创制项目（2013ZX09402203）：现代中药创新集群与数字制药技术平台，负责人：王振中。