目的：以人参、葛根药对为研究对象，分析人参、葛根单煎液与合煎液的化学成分变化。方法：Hypersil ODS（4.6 mm×250 mm，5μm）色谱柱；流动相为乙腈-水，梯度洗脱；检测波长为203 nm；柱温为25℃；流速为1 mL·min-1。结果：对人参、葛根合煎液HPLC 指纹图谱中主要峰进行了归属，经对比，人参、葛根单煎液与合煎液未见明显差异。结论：人参与葛根配伍后对化学成分含量无明显影响，其配伍原理可能与配伍前后有效成分吸收、代谢以及药效变化有关。

This study was aimed to analyze differences of chemical compounds of Ginseng and Radix Puerariae before and after compatibility using HPLC. Hypersil ODS column (4.6 mm × 250 mm, 5 μm) was adopted. The mobile phase was acetonitrile-water for gradient elution. The detection wavelength was set at 203 nm. The column temperature was 25℃. The flow rate was 1 mL/min. The results showed that through the study of all main peaks in the finger print spectra, there was no obvious influence on extract before and after compatibility of Ginseng and Radix Puerariae. It was concluded that there were no obvious chemical changes of Ginseng and Radix Puerariae before and after compatibility. The synergistic mechanism of compatibility might mainly come from the interaction between the pharmacological actions and the absorption or the metabolism of effective constituents of the medicinal plants.

科学技术部国家重大新药创制专项（2013ZX09402203）：现代中药创新集群与数字制药技术平台，负责人：王振中。