目的：探讨鼻敏感颗粒通过诱导Treg细胞变化，使变应性鼻炎的机体产生免疫耐受，达到治疗目的。方法：本研究以SD大鼠为实验动物，分为空白组、模型组和鼻敏感颗粒组，建立变应性鼻炎大鼠模型，并予以鼻敏感颗粒灌胃，借助流式细胞仪和ELISA法检测标本的 CD4+CD25+Foxp3+Treg和IL-4、IL-10、TGF-β1、IL-13的数值。结果：流式细胞仪检测结果表明，CD4+CD25+Foxp3+Treg在造模成功后下降（P<0.05），但在鼻敏感颗粒治疗后上升（P<0.05），达到正常空白组水平（P>0.05）。细胞因子ELISA法的结果表明，在造模成功后IL-10 和TGF-β1浓度明显下降（P<0.0001），IL-4和IL-13浓度明显上升（P<0.0001），而在鼻敏感颗粒治疗后IL-10和TGF-β1的血中浓度回升但未达到正常空白组水平，IL-4和IL-13血中浓度下降但未达到正常空白组水平。结论：鼻敏感颗粒可以上调变应性鼻炎大鼠的Treg细胞数量，使其分泌的IL-10 和TGF-β1量上升，抑制Th2细胞活性，使IL-4和IL-13分泌量下降，从而改善变应性鼻炎症状。

This study was aimed to explore Bi-Min-Gan (BMG) granules induced immune tolerance for allergic rhinitis cases through changes of Treg cells to achieve the goal of treatment. SD rats were used as experimental animal, which were divided into the blank group, model group and BMG granules group. Allergic rhinitis rat model was established. Intragastric administration of BMG granules was given. Levels of CD4+CD25+Foxp3+Treg and IL-4, IL-10, TGF- β1, IL-13 were detected with flow cytometry and ELISA assay. The results of flow cytometry showed that CD4+CD25+Foxp3+Treg decreased after the successful establishment of the model (P<0.05); but it increased after treatment of BMG granules (P<0.05) to reach the level of the normal blank group (P>0.05). ELISA showed the concentrations of IL-10 and TGF-β1 decreased significantly after the successful establishment of the model (P<0.0001); concentrations of IL-4 and IL-13 increased significantly (P<0.0001). While, after treatment of BMG granules, concentrations of IL-10 and TGF-β1 in blood rebound but did not reach the level of the normal blank group; concentrations of IL-4 and IL-13 in the blood decreased but did not reach the level of the normal blank group. It was concluded that BMG granules can upregulate the number of Treg cells in allergic rhinitis rat, increase the secretion of IL-10 and TGF-β1, inhibit Th2 cell activity, and reduce secretion of IL-4 and IL-13, so as to improve symptoms of allergic rhinitis.

江苏省中医药局2013年江苏省中医药局科技项目（LZ13055)：益气温阳法诱导变应性鼻炎Foxp3+Treg-免疫耐受途径的机制研究，负责人：史军；江苏省教育厅2013年度普通高校研究生科研创新计划项目（CXZZ13_0616）：益气温阳法诱导变应性鼻炎Treg-免疫耐受的机制，负责人：史军；江苏省中医院2013年江苏省中医院博士项目（K2013Y29）：鼻敏感颗粒调控变应性鼻炎Treg细胞诱导免疫耐受的研究，负责人：史军。