目的：观察铁皮石斛对冠心病模型家兔心功能和血管变化的影响。方法：清洁级健康家兔30只随机分为对照组、模型组、铁皮石斛低剂量、中剂量、高剂量组5组，每组6只。模型组、低剂量、中剂量、高剂量组采用左冠状动脉放置缩窄环方法，制备冠心病模型。低剂量、中剂量、高剂量组以铁皮石斛按10 g·kg-1、20 g·kg-1、40 g·kg-1灌胃（ig），每天1次，连续21天。对照组、模型组则给予等体积生理盐水。检测白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）、血管细胞间粘附分子-1（VCAM-1）、细胞间粘附分子-1（ICAM-1）水平、actin表达及心功能指标左室收缩压（LVSP）、左室舒张末压（LVEDP）、左室压力变化最大上升和下降速率 （±dp/dtmax）、动脉收缩压（BPs）和动脉舒张压（BPd）的变化并观察血管的形态学。结果：与对照组比较，模型组IL-8、TNF-α、VCAM-1、ICAM-1水平、actin表达升高或显著升高，差异有统计学意义（P<0.05或P<0.01）；LVEDP显著升高（P<0.01），而LVSP、±dp/dtmax、BPs均降低（P<0.05或P<0.01），血管壁变厚。与模型组比较，中、高剂量组IL-8、TNF-α、VCAM-1、ICAM-1水平、actin表达降低或显著降低，差异有统计学意义（P<0.05或P<0.01）；中、高剂量组LVEDP显著降低，差异有统计学意义（P<0.01），而LVSP、±dp/dtmax、BPs均升高或显著升高，差异有统计学意义（P<0.05或P<0.01），血管壁变厚得到改善。结论：铁皮石斛具有改善心功能作用，可能是通过改善和抑制血管病理改变、减轻血管损害而起作用。

This study was aimed to observe effects of Dendrobium officinale Kimura et Migo on cardiac function and changes of blood vessel in rabbits with coronary heart disease (CHD). Thirty clean grade healthy rabbits were randomly divided into 5 groups, which were the control group, model group, Dendrobium low-dose (DLD), middle-dose (DMD), and high-dose (DHD) group, with 6 rabbits in each group. Constrictor ring was implanted in the left coronary artery to establish CHD model in the model group, DLD, DMD and DHD groups. Intragastric administration of Dendrobium decoction at the dose of 10, 20, and 40 g·kg-1 was given to rabbits in the DLD, DMD and DHD groups once a day for 21 consecutive days, respectively. The same volume of normal saline was given to the control group and the model group. Detections were made on levels of IL-8, TNF-α, VCAM-1, ICAM-1, the expression of actin, changes of LVSP, LVEDP,±dp/dtmax, BPs, BPd and morphologic changes of blood vessel. The results showed that compared with the control group, levels of IL-8, TNF-α, VCAM-1, ICAM-1 and the expression of actin increased or significantly increased in the model group with statistical significance (P<0.05, or P<0.01); LVEDP increased significantly (P<0.01). However, LVSP,±dp/dtmax and BPs reduced (P<0.05, or P<0.01). The blood vessel wall became thicker. Compared with the model group, IL-8, TNF-α, VCAM-1, ICAM-1 and the expression of actin in the DMD and DHD group reduced or significantly reduced with statistical significance (P<0.05, or P<0.01); LVEDP reduced significantly in the DMD and DHD group with statistical significance (P<0.01). While, LVSP,±dp/dtmax and BPs increased or significantly increased with statistical significance (P<0.05, or P<0.01). The thickening of blood vessel wall was inhibited. It was concluded that Dendrobium officinale Kimura et Migo can improve cardiac function, which may take effect by improving and inhibiting pathologic changes of blood vessel and alleviating vessel injury.

广西科技厅广西科学研究与技术开发计划（桂科攻1347003-4）：冠心病血瘀证病证结合家兔动物模型的构建与中药干预其特征分子群的研究，负责人：唐汉庆。