目的：建立同时测定蒺藜中3种成分含量的方法，比较不同生长期、不同产地及不同药用部位的蒺藜中3种指标成分含量，探究采收时间及种植地区的科学性。方法：以蒺藜中3种有效成分，即槲皮素-3-O-β-吡喃葡萄糖基-(1→6)-吡喃葡萄糖苷、异鼠李素-3-O-β-D-吡喃葡萄糖基-(1→6)-吡喃葡萄糖苷、Terrestrinone A2为指标成分，高效液相色谱（HPLC）法测定含量。结果：3种指标成分含量均逐渐递增至6月并达到最大，随后逐渐降低；均表现为茎叶>果>根，根中含量低于检测限；产于内蒙古的蒺藜指标成分含量远远高于其他地区，其次为山东、河北、陕西。结论：该方法简便、准确，具有较好的重复性和稳定性，可为蒺藜质量控制提供参考。

This study was aimed to establish a method for the content determination of three components in Tribulus terrestris L., in order to compare index components of different growing periods, production places and medical parts for the scientific explanation of the harvesting time and growing places. Contents of three effective components in Tribulus terrestris L., which were quercetin-3-O-β-D-gentiobioside, isorhamnetin-3-O-β-D-gentiobioside and Terrestrinone A2, were determined by HPLC. The results showed that contents of three evaluation indexes all gradually increased until June and then started reducing. The contents in all evaluated indexes were in the order of the stem and leaf > fruit > root. And the content in the root was below the detection limit. The content indexes of Tribulus terrestris L. of the Inner Mongolia were much higher than that of other regions. It was followed by Shandong, Hebei and Shanxi province. It was concluded that the method was simple and accurate with good repeatability and stability, which was suitable for quality control of Tribulus terrestris L.

科学技术部国家国际科技合作项目（2010DFB33260）：中医药干预治胰岛素抵抗创新中药研究，负责人：徐暾海；北京中医药大学创新团队项目（2011-CXTD-19）：中医药防治糖尿病研究团队，负责人：刘铜华；2011北京市教委共建项目：中医药干预胰岛素抵抗技术平台建设与方药筛选研究，负责人：刘铜华。