目的：探索姜黄素与中低剂量化疗药5-氟尿嘧啶（5-FU）联用对胃癌MGC-803细胞生长、细胞凋亡和细胞周期的影响，为临床上应用姜黄素作为胃癌化疗药5-FU的增效剂，以减少5-FU使用剂量、降低其毒副作用提供实验依据。方法：应用MTT检测法检测药物作用对MGC-803细胞的抑制作用；应用基于AO/EB双染的荧光显微观察法和FITC/PI双染的流式细胞技术检测药物作用对MGC-803细胞凋亡的影响；应用基于PI单染的流式细胞技术检测药物作用对MGC-803细胞周期的影响。结果：姜黄素（25 μmol·L^-1）分别与低剂量（2.4 μmol·L^-1）和中剂量（4.8 μmol·L^-1）的5-FU联合作用能有效地抑制MGC-803细胞的生长，诱导细胞凋亡并将细胞周期阻滞在S期，并呈剂量-时间的依赖关系。姜黄素与低剂量5-FU联用组的效果显著优于中剂量（4.8 μmol·L^-1）5-FU单用组（P < 0.01），而姜黄素与中剂量5-FU联用组的效果显著优于高剂量（9.6 μmol·L^-1）5-FU单用组（P< 0.01），提示姜黄素能显著增强5-FU抗胃癌MGC-803细胞的作用，并呈剂量-时间的依赖关系。结论：本研究为临床上应用姜黄素作为5-FU治疗胃癌的辅助用药，以减少5-FU的使用剂量，从而降低其毒副作用提供了初步的实验依据。

This study was aimed to provide experimental evidence for clinical application of curcumin to strengthen the effect of 5-Fluorouracil (5-FU) in gastric cancer treatment, so as to reduce the dose of 5-FU and its side-effect in the future treatment. MTT assay was used to detect medication effect on MGC-803 cell growth inhibition; AO/EB double staining fluorescence microscopic observation and FITC/PI double staining flow cytometry were used to detect medication effect on cell apoptosis. Flow cytometry was based on PI staining used to detect medication effect on MGC-803 cell cycle. The results showed that curcumin (25 μmol·L^-1) with low-dose (2.4 μmol·L^-1) and middle-dose (4.8 μmol·L^-1) 5-FU can effectively inhibit MGC-803 cell growth, induce cell apoptosis and block cell cycle in S phase. Which showed dose-time dependent manner. The combined use of curcumin with low-dose 5-FU was more effective than the middle-dose (4.8 μmol·L^-1) 5-FU alone (P < 0.01); similarly, the combined use of curcumin with middle-dose 5-FU was more effective than the use of high-dose (9.6 μmol·L^-1) 5-FU alone (P < 0.01). It indicated that curcumin can enhance the antitumor effect of 5-FU against MGC-803 cells in a dose-time dependent manner. It was concluded that the study provided a preliminary experimental basis for using curcumin as an adjuvant to 5-FU with the benefit of reducing its dose and toxicity in the clinical treatment of gastric cancer.

湖北省科技厅自然科学基金面上项目（2013CFB067）：姜黄素联合氟尿嘧啶诱导胃癌细胞凋亡的效应与机理研究，负责人：赵刚。