目的：从桑寄生中提取高质量的RNA。方法：以桑寄生叶片、茎、花和种子为材料，采用TRIzol试剂盒法、改良TRIzol法、CTAB-LiCl法、CTAB-异丙醇法4种方法，比较不同材料及不同方法分离RNA的效果。结果：TRIzol试剂盒法和改良TRIzol法均不能有效的去除桑寄生中的多糖和蛋白质等杂质，不适合用于桑寄生RNA的提取；CTAB-LiCl法提取的RNA质量较高、完整性较好,可满足各种后续分子生物学实验的要求；CTAB-异丙醇法提取桑寄生RNA的效果虽然较好，但还达不到小RNA分析实验要求。结论：为桑寄生后续分子生物学研究提供基础，也为次生代谢物较丰富的药用植物RNA的提取提供参考。

This study was aimed to extract high quality RNA from the T.chinensis(DC.)Danser.The leaves,stems,flowers and seeds of T.chinensis(DC.)Danser were used as materials.Four methods,which were the TRIzol reagent kit method,improved TRIzol method,CTAB-LiCl method,and CTAB-isopropyl alcohol method,were used to compare the effect of different methods and materials for the separation of RNA.The results showed that TRIzol reagent kit method and the improved TRIzol method cannot effectively remove the impurities such as polysaccharide and protein in the T.chinensis(DC.)Danser.Both of them were not suitable for the extraction of RNA from T.chinensis(DC.)Danser.The extraction of RNA with CTAB-LiCl method was with integrity and high quality,which can meet the requirements of various molecular biology experiments subsequently.The RNA extraction effect with the CTAB-isopropyl alcohol method from T.chinensis(DC.)Danser was better,but it still cannot meet the small RNA analysis experiment requirement.It was concluded that the study provided foundation for T.chinensis(DC.)Danser subsequent molecular biology research,as well as provided the reference for the extraction of RNA from medicinal plants with relatively rich secondary metabolites.

国家自然科学基金委青年科学基金项目（81403045）：桑寄生顽拗性种子保存及脱水敏感性死亡机理研究，负责人：韦树根；广西壮族自治区科学技术厅青年科学基金（2014GXNSFBA118204）：桑寄生顽拗性种子脱水敏感性分子机理研究，负责人：潘丽梅；科学技术部“重大新药创制”科技重大专项（2012ZX09304006）：中药材种子种苗和种植（养殖）标准平台子课题——云木香、桑寄生、草果种子质量标准研究，负责人：马小军。