目的：基于核磁共振氢谱代谢组学技术（¹H-NMR）探讨艾灸与针刺对健康青年志愿者血清代谢物调节作用的差异性，从代谢物层面揭示两者的作用特点及差异。方法：将60例健康青年男性志愿者随机分为艾灸组与针刺组，每组各30例，艾灸组采用温和灸作用于右侧足三里穴，每天1次，每次15min，连续干预10天；针刺组采用不锈钢针针刺右侧足三里穴，每天1次，每次15min，连续干预10天。两组在干预0、5、10天清晨采集受试者血清，利用¹H-NMR技术对血清代谢物进行检测。结果：干预0、5天时艾灸组与针刺组的代谢模式无显著性差异；干预10天时艾灸组与针刺组的代谢模式可显著区分；艾灸与针刺干预5天后血清中表现出差异的代谢物有Creatine和Glycine（P<0.05；P<0.01）；干预10天后血清中表现出差异的代谢物有LDL/VLDL、Valine、Isoleucine、Leucine、Lactate、Glutamine、Creatine、Glucose（P<0.01）和Glycine（P<0.05）；随着干预时间的变化，艾灸与针刺对血清代谢物的调节作用有差异。结论：艾灸与针刺右侧足三里对血清代谢物有明显的调节作用，随着干预时间的变化，两者的调节作用发生变化。

This study was aimed to reveal the differences of metabolites between moxibustion and acupuncture on serum based on ¹H-NMR.A total of 60 healthy young male volunteers were randomly divided into the moxibustion group and the acupuncture group,with 30 cases in each group.In the moxibustion group,the treatment was mild moxibustion on the right“Zusanli”point for 15 minutes,once a day for 10 days.In the acupuncture group,acupuncture was used on the right“Zusanli”point for 15 minutes,once a day for 10 days.Serum was collected on the morning of 0-day,5-day,and 10-day.The result showed that there was no significant difference on the metabonomic pattern on the 0-day and 5-day between the moxibustion group and the acupuncture group.After 10-day intervention,there were significant changes on the metabonomic pattern between the moxibustion group and the acupuncture group.After 5-day intervention,2 metabolites changed significantly(creatine and glycine)(P<0.05, P<0.01).After 10-day intervention,9 metabolites changed significantly(LDL/VLDL,valine,isoleucine,leucine,lactate,glutamine,creatine and glucose (P<0.01),and glycine(P<0.05).It was concluded that moxibustion and acupuncture had obvious adjustment to the metabolites,along with the treatment time indicated the regulation function changed.

科学技术部国家重点基础研究发展计划“973计划”项目（2015CB554502）：艾灸与针刺作用特点、效应响应异同的基础与临床研究，负责人：常小荣；国家自然科学基金委面上项目（81574082）：基于COX-2及相关信号通路研究针灸对慢性萎缩性胃炎大鼠的胃黏膜保护机制，负责人：刘密；高等学校博士学科点专项科研基金新教师类资助课题（20124323120002）：基于TFF/PAR介导的ERK信号转导途径研究艾灸促进胃黏膜损伤修复的作用机制，负责人：刘密；湖南省教育厅湖南省优秀博士学位论文获奖作者科研资助项目（YB2013B037）：合募配穴艾灸对胃黏膜损伤修复过程中三叶因子的调节作用，负责人：刘密；湖南省教育厅资助科研项目（14B129）：基于下丘脑弓状核-外侧隔核Ghrelin通路研究针灸对糖尿病大鼠胃运动的调节机制，负责人：刘密；湖南省教育厅湖南省研究生科研创新项目（CX2015B320）：基于代谢组学方法探讨针刺与艾灸对刺激点的局部作用机制，负责人：钟欢。