目的：以斑马鱼为模型，探讨马钱子的抗血管生成活性。方法：以Tg（kdrl:mCherry）斑马鱼和野生斑马鱼为模型，在安全浓度范围内，分别采用荧光显像和体内碱性磷酸酶定量检测的方法，以PTK787为阳性对照，考察马钱子水提取物对斑马鱼血管生成的抑制作用。结果：与空白对照组比较，在一定的浓度范围内，低浓度的马钱子水提取物能抑制Tg（kdrl:mCherry）斑马鱼的节间血管生成，随着提取物浓度的增加抑制作用更加明显，抑制作用呈剂量依赖性。在碱性磷酸酶定量检测实验中，马钱子水提取物浓度为25μg·mL^-1时，血管生成抑制率为8.61%；浓度为100μg·mL^-1时，血管生成抑制率为26.16%；在一定的浓度范围内，马钱子处理组均表现出显著的抗血管生成活性（P<0.05），血管生成抑制率与马钱子水提取物浓度成线性关系。结论：马钱子水提取物可以有效的抑制斑马鱼血管生成，有明显的抗血管生成活性。

This study aimed to explore the anti-angiogenetic effect of the seeds of S.nux-vomica on zebrafish.Taking Tg(kdrl:mCherry) zebrafish and wild zebrafish as the models,fluorescence imaging and quantitative endogenous alkaline phosphatase assay in vivo respectively were adopted in the scope of the safe concentr ation of S.nux-vomica seeds.The angiogenesis activity of S.nux-vomica seeds in zebrafish was investigated taking PTK787 as the positive control.As a result,in a certain range of concentrations,the low concentration of the water extract of S.nux-vomica seeds limited the number of intersegmental blood vessels in Tg(kdrl:mCherry)zebrafish compared with the blank control group.With the increased concentration of the water extract of S.nux-vomica seeds,the anti-angiogenetic effect enhanced in a dose-dependent manner.The quantitative endogenous alkaline phosphatase assay showed that as the concentration of water ext ract of the seeds of S.nux-vomica was 25μg·mL^-1the anti-angiogenesis effect was 8.61%;and while the concentration was 100μg·mL^-1,the anti-angiogenesis effect was 26.16%.At least for some range of concentrations,the different treatment groups showed that the difference was statistically significant(P<0.05),and the anti-angiogenetic effect was closely associated with the concentration of the water extract of S.nux-vomica seeds in the linear relation.In conclusion,the water extract of S. nux-vomica seeds presented significant anti-angiogenetic effect and effectively suppressed angiogenesis in zebrafish.

北京市教委共建项目：基于化学成分和斑马鱼特性对有毒中药材进行安全性评价的关键技术研究和应用，负责人：林瑞超。