目的：利用代谢组学技术，结合临床生化检测和组织病理学，评价茵陈蒿汤及其组分配伍对长期过量摄入酒精诱导肝损伤的保护作用。方法：对Wistar大鼠进行“白酒—玉米油—吡唑”混合溶液灌胃和饲喂高脂饲料12周制备酒精性肝损伤大鼠模型，茵陈蒿汤及其组分配伍组分别于造模开始每日灌胃给予茵陈蒿汤和6,7-二甲氧基香豆素、京尼平苷、大黄酸配伍组合，12周末测定血清ALT、AST、ALP、TG、T-BIL、TP、ALB、CHOL、HA、LN、PCIIINP、CIV浓度和肝脏组织HYP、γ-GT、MDA、TBA、SOD和GSHPX含量，观察肝左叶HE和Masson染色病理组织切片，并利用UPLC-Q/TOF-MS技术采集尿液代谢轮廓数据，提取各组样品中酒精性肝损伤生物标记物信息，分析空白对照组、模型对照组、茵陈蒿汤组及茵陈蒿汤组分配伍组之间大鼠代谢轮廓及生物标记物的变化。结果：茵陈蒿汤及其组分配伍均能显著抑制酒精性肝损伤大鼠血清ALT、AST、ALP、TG、T-BIL、TP、ALB、CHOL、HA、LN、PCIIINP、CIV浓度和肝脏组织HYP、γ-GT、MDA、TBA、SOD和GSH-PX含量的变化，减少肝脏细胞炎性浸润和肝纤维化，并使肝损伤大鼠整体代谢轮廓远离模型对照组而靠近正常对照组，使10个生物标记物趋于正常水平。结论 茵陈蒿汤对大鼠酒精性肝损伤具有显著的保护作用，其3个组分6,7-二甲氧基香豆素、京尼平苷和大黄酸的配伍组合能够有效表达茵陈蒿汤的生物效应，是其主要有效组分，为茵陈蒿汤防治酒精性肝损伤疾病的创新药物开发奠定了基础。

The present investigation was designed to evaluate the protective effects of Yin Chen Hao Decoction(YCHD)and its component group consisting of scoparone,geniposide and rhein on rats with liver injury induced by long-term excessive intake of alcohol using metabonomic technologies and typical pharmacodynamics indexes.Wistar rats had been administered with the mixed solution of alcohol,corn oil and pyrazole and high fat diet for 12 weeks to establish the alcoholic liver injury rat model.At the start day of modeling,rats were intragastricly administered with YCHD and its component group involving scoparone,geniposide and rhein.At the end of the 12th week,the serum concentrations of ALT,AST,ALP,TG,T-BIL,TP,ALB,CHOL,HA,LN,PCIIINP and CIV were detected and so were the contents of HYP,γ-GT,MDA,TBA,SOD and GSH-PX in the liver.Meanwhile,the pathological examinations of left lobe of liver sections were implemented with HE and Masson staining.Finally,urine endogenic metabolites profiling data and vital biological information of alcoholic liver injury were gleaned using UPLC-Q/TOF-MS-based metabonomic technology.The regulations of biomarkers in the control group,the model group,the YCHD group and the component group were quantified with the pattern recognition and statistics technology.As a result,YCHD and its component group successfully suppressed the changes of ALT,AST,ALP,TG,T-BIL,TP,ALB,CHOL,HA,LN,PCIIINP,CIA,Hyp,γ-GT,MDA,TBA,SOD and GSH-Px with the long-term excessive intake of alcohol.Liver inflammatory cell infiltration and liver fibrosis were improved,while the metabolic profile was far from the model group but close to the control group.Among the 21 biomarkers of alcoholic liver injury rats,10 biomarkers recovered to normal levels.In conclusion,YCHD presented favorable effects on liver injury rats induced by the long-term excessive intake of alcohol.The combination of scoparone,geniposide and rhein played a representative biological role in the treatment of YCHD,which laid a foundation for the development of some innovative drugs of alcohol liver injury.

国家自然科学基金委重点项目（81430093）：中药体内药效物质基础的系统分析方法学——中医方证代谢组学研究，负责人：王喜军；科学技术部“重大新药创制”国家科技重大专项（2015ZX09101043-011）：中药经典名方整合作用机制关键技术研究，负责人：王喜军。