目的：以巴西龟为来源的龟甲胶中牛皮特征肽成分的检测。方法：采用胰蛋白酶进行酶解，利用超高效液相色谱-电喷雾四极杆飞行时间质谱法分别对以巴西龟为来源的龟甲胶和以中华草龟为来源的龟甲胶进行测定，选定牛皮特征肽离子m/z 604.8、m/z 641.3、m/z 790.8作为检测对象。结果：巴西龟来源的龟甲胶中只检测出牛皮特征肽离子m/z 641.3信号，其MS/MS裂解规律与黄明胶标准品酶解产物所含牛皮特征肽GEAGPSGPAGPTGAR一致，而中华草龟来源的龟甲胶中均未检测出牛皮特征肽信号。结论：该方法专属性强，灵敏度高，可区别巴西龟龟甲胶和掺杂牛皮成分的龟甲胶，可用于龟甲胶的质量控制。

This study aimed at developing an ultra-performance liquid chromatography electrospray time of flight-mass spectrometry(UPLC-ESI-QTOF-MS)method for the identification of bovine hide gelatine from the shell glue of the red-eared slider.Tortoise shell glue was made from the shell of red-eared slider or Reeves'turtle(Chinemys reevesii).The samples were analyzed on UPLC-ESI-QTOF-MS after being digested by trypsin,and the ions at m/z 604.8,m/z 641.3,m/z 790.8 of the marker peptides of bovine hide gelatin were detected.As a result,it was found that the test results of the ions made from the shell of red-eared slider at m/z 641.3 were positive,taking the same fragmentation regularity of MS/MS as the standard of bovine-hide gelatine,while negative results were detected from the ions made from the shell of Reeves'turtle.In conclusion,the method was simple and accurate provided a basis for distinguishing the tortoise shell glue from bovine hide,which could be also used for the quality control of tortoise shell glue.

国家中医药管理局“药用植物学”重点学科课题（国中医药发[2009]30号），负责人：李顺祥；湖南省教育厅“中药学”重点学科（湘教通[2011]76号），负责人：李顺祥；湖南省教育厅高校科技创新团队（湘教通[2010]212号），负责人：李顺祥。