目的：利用代谢组学技术检测冠心病心绞痛中医证候中的潜在生物标志物，探讨冠心病心绞痛不同证型的客观化规律。方法：选取健康组、冠心病心绞痛患者气虚血瘀证和气虚血瘀痰浊证，应用液相色谱-质谱联用（LC-MS）的方法，检测健康组和不同中医证型的血清、尿液的内源性代谢物，通过主成分分析（PCA)和正交信号校正偏最小二乘判别分析（OPLS-DA）的方法分析其代谢谱。结果：在PCA分析中，健康组和气虚血瘀组、气虚血瘀痰浊组之间可以明显的区分开来；气虚血瘀证型的潜在生物标志物有天冬氨酰蛋氨酸、半胱氨酸亚磺酸；气虚血瘀痰浊证型的潜在生物标志物有马尿酸、氨基葡萄糖、果糖胺、甘油三酯；气虚证型客观化的表现为缺少生物素、赖氨酰酪氨酸、磷脂酰甘油、甘氨胆酸。结论：通过代谢组学这项新技术，探讨出冠心病心绞痛气虚血瘀证型、气虚血瘀痰浊证型的不同内源性代谢产物，为临床不同中医证型的预防、诊治提供思路。

This study was aimed to investigate potential biomarkers in different traditional Chinese medicine (TCM) syndromes of angina pectoris of coronary heart disease (CHD) by using metabolomic technology, and to explore the objective law of different TCM syndromes of CHD. Endogenous metabolites in serum and urine from the healthy group, and patients with DHD angina pectoris patients of the syndrome of Qi deficiency and blood stasis, as well as the syndrome of Qi deficiency, blood stasis and phlegm turbidity were detected by the liquid chromatography - mass spectrometry (LC-MS). Metabolic profiles were analyzed by the principal component analysis (PCA) and the orthogonal partial least squares discriminant analysis (OPLS-DA). The results showed that in PCA, the healthy group, Qi deficiency and blood stasis group, as well as the Qi deficiency, blood stasis and phlegm turbidity group can be obviously distinguished. Potential biomarkers in the Qi deficiency and blood stasis syndrome contained aspartyl methionine and cysteine sulfinic acid. Potential biomarkers in the Qi deficiency, blood stasis and phlegm turbidity syndrome contained hippuric acid, amino glucose, fructosamine and triglyceride. The objective performance of Qi deficiency syndrome was the absence of biotin, lysyl tyrosine, phosphatidylglycerol and glycocholic acid. It was concluded that through the new metabolomic technology, different endogenous metabolites in the syndrome of Qi deficiency and blood stasis, as well as the syndrome of Qi deficiency, blood stasis and phlegm turbidity of patients with DHD angina pectoris were detected. It provided ideas for the clinical practice of prevention, diagnosis and treatment of different TCM syndromes.

国家中医药管理局国家中医临床研究基地业务建设科研专项课题（JDZX2012070）：冠心病中医证候与伏邪发病机制的流行病学调查及代谢组学研究，负责人：邓悦。