目的：探讨甲炎康泰对自身免疫性甲状腺炎（AIT）大鼠Th1/Th2细胞平衡偏移作用机制。方法：采用Lewis大鼠建立AIT大鼠40只，6周后随机分为模型组、甲炎康泰低、中、高剂量组，每组10只，每天分别按去离子水、0.708、1.417、2.834 g ? kg -1 剂量连续灌胃8周。检测血清TgAb、TPOAb；Th1、Th2细胞分布比例及Th1/Th2比率；检测血浆中IL-4、IL-5、IFN-γ、TNF-α和IL-1α含量；HE染色观察甲状腺组织的病理；免疫组织化学检测甲状腺IFN-γ、IL-4蛋白表达。结果：甲炎康泰高剂量组能够下降AIT大鼠TPOAb、TGAb水平，降低甲状腺炎发生率、Th1与Th2细胞分布比例以及Th1/Th2比率；降低血浆IFN-γ、TNF-α、IL-1α、IL-4、IL-5含量；降低甲状腺IFN-γ、IL-4蛋白表达。结论：甲炎康泰具有降低AIT大鼠甲状腺自身抗体的作用，可能与通过调节细胞因子水平和Th1/Th2细胞平衡偏移有关。

This study was aimed to explore the mechanism of Jia-Yan-Kang-Tai (JYKT) on Th1/Th2 immune cell imbalance in rats with autoimmune thyroiditis (AIT). A total of 40 Lewis rats were made into AIT rat model. Six week later, AIT rats were randomly divided into the model group, low-dose, middle-dose and high-dose JYKT group, with 10 rats in each group. Intragastric administration of deionized water, 0.708 g ? kg -1 , 1.417 g ? kg -1 and 2.834 g ? kg -1 JYKT was given to rats in each group, respectively for 8 consecutive weeks. Serum TgAb and TPOAb were detected. The ratio ofTh1 cell, Th2 cell and Th1/Th2 was measured. Contents of IL-4, IL-5, IFN-γ, TNF-α and IL-1α in plasma were detected. The pathology of thyroid tissues was observed by HE dyeing. The protein expressions of IFN-γ and IL-4 in thyroid tissues were observed under immunohistochemical staining. The results showed that high-dose JYKT group can reduce the level of TPOAb and TGAb, the incidence of thyroiditis, the ratio of Th1 cell, Th2 cell and Th1/Th2; decrease contents of IFN-γ, TNF-α, IL-1α, IL-4 and IL-5 in plasma; reduce the protein expression of IFN-γ and IL-4 in thyroid tissues. It was concluded that JYKT was able to suppress thyroid autoantibodies of AIT rats, which may be related to the regulation of cytokine level and Th1/Th2 immune cell imbalance.

科学技术部国家国际科技合作专项项目（2015DFA30910）：中药干预桥本氏甲状腺炎活性筛选技术与组方优化研究，负责人：刘铜华。