目的：探讨生脉注射液对心肌缺血Beagle犬血压、心率、心电图及血清C-反应蛋白、NO水平的影响。方法：30只Beagle犬随机分为5组，即空白对照组、模型对照组、生脉注射液小剂量组(0.80 mL·kg -1 ）、生脉注射液大剂量组（3.20 mL·kg -1 ）和单硝酸异山梨酯组（0.07 mL·kg -1 ）。采用皮下注射盐酸异丙肾上腺素制备心肌缺血模型，同时给予药物治疗。记录给药后1、6、12、24 h，犬血压、心率和心电图变化，并检测血清C-反应蛋白和NO含量。结果：与空白对照组比较，模型组Beagle犬平均动脉压（MBP）明显降低（P<0.01），心率（HR）明显加快（P<0.01或P<0.05），心电图ΔST值明显增大（P<0.01），生脉注射液能改善上述症状，但作用趋缓。生脉注射液对血清C-反应蛋白含量降低和NO含量升高有明显的对抗作用，与模型组比较，差异有统计学意义（P<0.01或P<0.05）。结论：生脉注射液能改善心肌缺血Beagle犬生理指标，其机制可能与调节C-反应蛋白代谢，抑制NO分泌有关。

This study was aimed to explore the effects of Shengmai(SM) injection on blood pressure (BP), heart rate (HR),electrocardiogram (ECG), and serum C-reactive protein (CRP) and NO levelin Beagle dogs with myocardial ischemia(MI). A total of 30 Beagle dogs were divided into 5 groups, which were the blank control group, model control group, SM injection low-dose group (0.80 mL·kg -1 ), SM injection high-dose group (3.20 mL·kg -1 ) and isosorbidemononitrate group(0.07 mL·kg -1 ). The MI model was established by the subcutaneous injection of isoprenaline hydrochloride, and the drug treatments were given at the same time. At 1 h, 6 h, 12 h, 24 h after administration, changes of BP, HR and ECG were recorded; serum CRP and NO content were detected. The results showed that compared with the blank control group, the mean arterial pressure (MBP) was significantly decreased (P<0.01), HR was significantly increased (P<0.01, or P<0.05), the ECGΔST value was significantly increased (P<0.01) in the model group. SM injection could improve symptoms mentioned above, but the effectswereslow. SM injection had a significant antagonistic effect on the decreasing of CRP content and the increasing of NO content, compared with the model group. The difference was statistically significant (P<0.01, or P<0.05). It was concluded that SM injection could improve the physiological indices of Beagle dogs with MI,which might be related to the regulation of CRP and the inhibition of NO secretion.

科学技术部国家"十二五"中药新药临床评价研究技术平台建设（2012ZX09303-017-001）：中药新药Ⅰ期临床关键技术和方法的研究，负责人：李国信。