目的：分离猫眼草乙酸乙酯部分提取物，对其指标成分进行含量测定，并对其抗肿瘤活性进行体外细胞实验研究，以期为猫眼草的利用与开发提供理论基础，为开发天然的抗肿瘤药提供新的方向和更多的选择。方法：高效液相色谱法测定乙酸乙酯部分槲皮素与没食子酸的含量；溶剂洗脱法对猫眼草乙酸乙酯部分进行分离；MTT法测定不同浓度的猫眼草乙酸乙酯各组份对乳腺癌ZR-75-30细胞生长的抑制作用。结果：猫眼草乙酸乙酯部分含有槲皮素19.99%，没食子酸55.04%。从猫眼草乙酸乙酯部分得到17个流份合并为6个组份。猫眼草乙酸乙酯部分分离的各组分（浓度50 μg·ml-1-150 μg·ml-1范围）对乳腺癌细胞ZR-75-30的生长有抑制作用，且呈现一定的梯度关系，随着药物浓度的升高，对乳腺癌细胞的生长抑制率增强。其中组分2的抗肿瘤活性最强。结论：猫眼草乙酸乙酯部分提取物含有黄酮和酚酸类成分，各组分对乳腺癌细胞生长均有抑制作用。

The ethyl acetate extract of Euphorbia lunulata Bge was separated and researched on anticancer activity aboutit with in vitro cell experiments, in order to provide a theoretical basis for the exploit and utilization of E. lunulata andmore options to develop natural anticancer drugs. Quercetin and Gallic Acid in ethyl acetate were extracted by HPLC,which the ethyl acetate extract of E. lunulata was separated and extracted by solvent system. The MTT was used tomeasure the inhibition of each component with different concentration from ethyl acetate extract to the 2R-75-30 breastcancer cell. The results showed that Quercetin and Galic Acid of ethyl acetate extract were 19.99% and 55.04% ,respectively. There were 17 component from the ethyl acetate extract, and finally merged into six fractions. Eachcomponent (the concentration of 50 μg/ml-150 μg/ml range) from ethyl acetate extract of E. lunulata could inhibit thegrowth of ZR-75-30 breast cancer cell. And a certain gradient relationship with the drug concentration increased withthe inhibition rate enhancement on the 2R-75-30 breast cancer cell?? s growth. The 2nd fraction showed the highestactivity. It was concluded that there were flavonoids and phenolic acids in ethyl acetate extract. Each component from theextract showed inhibition on the growth of breast cancer cell if it was further separated. This study was designed toprovide a theoretical basis to exploit the anticancer drugs from E. lunulata.

国家自然科学基金（81603265）：基于炎症和NF-kβ信号通路的重楼皂苷抗肿瘤体内药效物质基础及作用机制研究，负责人：何昊；陕西省中医 药管理局科研课题：基于“峻下-消肿”理论对猫眼草诱导肝癌细胞凋亡的药效物质基础研究（JCMS024），负责人：张彦；西安医学院国基金培育 项目（2016GJFY07）：基于EGFR靶点的木鳖子抗乳腺癌活性成分及作用机制研究，负责人：郑蕾；西安医学院国基金培育项目（2016GJFY06）： 基于VEGF/KDR通路的黄芩苷抑制肿瘤血管新生作用机制研究，负责人：申旭霁；西安医学院2016年大学生创新基金项目（2016DXS1-18）： 基于psbA-trnH基因片段的大戟属植物的DNA鉴定研究，负责人：刘强；陕西省大学生创新训练计划S201711840023基于rDNAd的ITS2条形 码序列鉴定大戟属药用植物，负责人：梁晶晶。