目的建立照山白浸膏片的TLC鉴别方法和HPLC特征图谱，为科学评价和有效控制其质量提供可靠方法。方法采用2种展开系统建立照山白浸膏片中金丝桃苷、槲皮素和山柰素的薄层色谱鉴别方法。采用HPLC法建立液相特征图谱：以金丝桃苷为参照峰，采用C18色谱柱（250×4.6 mm，5 μm）；以乙腈（A）-0.1%磷酸溶液（B）为流动相，进行梯度洗脱（0-13 min，8% A；13-28 min，8% A→21% A；28-46 min，21% A；46-60 min，21% A→40% A；60-77 min，40% A；77-80 min，8% A）；流速：1 ml?min-1；检测波长为366 nm；进样量：10 μl；柱温：30℃。结果鉴别分离良好。特征图谱有12个共有峰,在HPLC条件下均分离良好。结论所建方法可实现全面和整体的评价，为有效提高照山白浸膏片质量控制水平提供依据。

This study was aimed to establish the TLC identification method and HPLC characteristic chromatograms of Zhaoshanbai extract tablet, so as to provide a reliable method for scientific evaluation and effective quality control of this Chinese patent drug. Two kinds of phase system were adopted to establish the TLC for hyperin, quercetin and kaempferol of Zhaoshanbai extract tablet. The HPLC method was used to establish the liquid characteristic chromatogram of Zhaoshanbai extract tablet: with the peak of hyperoside as reference, ten samples were determined by HPLC method on a C18 column (250 mm × 4.6 mm, 5 μm) under the chromatographic conditions with acetonitrile (A) - 0.1% phosphoric acid solution (B) as gradient elution (0-13 min, 8%A; 13-28 min, 8%A→21%A; 28-46 min, 21%A; 46-60 min, 21% A→40%A; 60-77 min, 40%A; 77-80 min, 8%A) at a flow rate of 1.0 ml?min-1. The detection wavelength was 366 nm. The sample volume was 10 μl. The column temperature was 30℃ . The results showed that the sample was well indentified and separated. The HPLC specific chromatogram of Zhaoshanbai extract tablet had 12 common peaks. The peaks could be detected respectively. It was concluded that the established methods can realize a comprehensive and overall assessment, and provide a basis for improving the quality control of Zhaoshanbai extract tablet.

承德市科技局承德市科技研究与发展计划项目（201701A014）：照山白浸膏片质量控制方法的研究，负责人：崔小丽。