目的：优化参芪解郁处方的提取工艺。方法：用高效液相色谱（High Performance Liquid Chromatography， HPLC）法测定参芪解郁方传统汤剂以及颗粒剂中橙皮苷的含量，以煎煮时间、煎煮次数和加水量为考察因素，采用L9(34)正交试验确定最佳提取条件。结果：参芪解郁处方传统汤剂中橙皮苷含量显著高于颗粒剂中橙皮苷含量；汤剂中对橙皮苷提取量影响顺序为煎煮次数> 煎煮时间> 加水量，其中煎煮次数有显著性差异；参芪解郁方最佳煎煮条件为煎煮3次，煎煮0.5小时，加水量7倍。结论：参芪解郁方高效液相分析方法为该 方的定量方法；优选的参芪解郁方煎煮工艺稳定，提取率高。

Objective: To optimize the extraction technology of Shen Qi Jie Yu prescription. Methods: High performance liquid chromatography (HPLC) method was selected to determine the content of hesperidin of Shen Qi Jie Yu prescription of traditional Tang and granules. Decoction time, times and add water were used as the examining factors. L9(34) orthogonal test was used as the best condition of extraction. Results: The hesperidin content in traditional Tang of Shen Qi Jie Yu prescription was significantly higher than granules. The order of the effect of hesperidin content is decoction time> decoction times >add water. There is a significant difference in the number of boiling times. The best decoction condition of Shen Qi Jie Yu Prescription was 3 times for decoction, with 0.5 hours of decoction and 7 times of water addition. Conclusion: The high performance liquid chromatography of Shen Qi Jie Yu Prescription is the quantitative method and the preferred decoction technique of Shen Qi Jie Yu prescription is stable and reliable.

中医药管理局脑病科重点专科建设项目，负责人：孙文军；北京中医药大学中央高校基本科研业务费专项资金（2016-JYB-JSMS-061）：参芪解郁方调控母婴分离产后抑郁大鼠5-羟色胺的药效物质基础研究，负责人：曲淼。