目的：探讨音乐电针和脉冲电针对快速老龄化（SAMP8）小鼠行为学和海马星形胶质细胞的影响，对比观察其抗痴呆的作用机制。方法：8月龄雄性SAMP8小鼠共30只，随机分为模型组，音乐电针组，脉冲电针组（n=10），以同源的正常老化（SAMR1）小鼠为空白组（n=10），选取“百会”、“印堂”、“人中”，每天针刺干预1次，共15天。采用Morris水迷宫观察各组小鼠学习记忆能力变化，运用免疫组化法观察海马GFAP的表达， Western blot检测海马GFAP的蛋白水平。结果：Morris水迷宫提示：与空白组相比，模型组的逃避潜伏期延长 （P < 0.01），空间探索实验游泳距离增长（P < 0.01）；与模型组相比，音乐电针组和脉冲电针组逃避潜伏期缩短（P < 0.01，P < 0.05），游泳路程缩短（均P < 0.05）。免疫组化结果：与空白组相比，模型组海马CA1区GFAP平均光密度明显增多（P < 0.01）；与模型组相比，音乐电针组和脉冲电针组海马CA1区GFAP平均光密度均明显降低（均P < 0.01），且音乐电针组GFAP平均光密度低于脉冲电针组（P < 0.05）。Western blot检测：与空白组相 比，模型组海马GFAP蛋白表达明显增多（P < 0.01）；与模型组相比，音乐电针组和脉冲电针组GFAP蛋白表达有明显变浅和下降趋势（均P < 0.05）；音乐电针组GFAP蛋白表达明显低于脉冲电针组（P < 0.05）。结论：音乐电针和脉冲电针均可改善SAMP8小鼠学习记忆能力，降低GFAP的表达，可能与其减轻神经炎性反应、改善凋亡，最终达到保护神经元的机制有关，且音乐电针有优于脉冲电针的趋势。

Objective: To observe the effects of music electro-acupuncture and pulsed electro-acupuncture on locomotor avtivity and hippocampal astrocytes in fast- aging (SAMP8) mice, and to compare the anti- dementia mechanism.Methods: Thirty 8-month-old male SAMP8 mice were randomly divided into model group(group M), music electroacupuncturegroup(group MA), pulsed electro- acupuncture group(group EA) (n=10), with homologous normal agingSAMR1 mice as control group(group C)(n=10). Acupuncture stimulation was applied to“Baihui”(GV20)、“Yintang”(GV29) and“Renzhong”(DU26) for 20 min per day for 15 days. The Morris water maze was used to assess learningmemorizeability. Immunohistochemical DAB staining was used to observe GFAP exptession in hippocampus. DetectGFAP protein levels by Western blot in hippocampus. Results: The Morris water maze test showed : Compared with groupC, the escape latency were increased (P < 0.01), swimming distance were increased in space exploration experiment in group M (P < 0.01). Compared with group M, the escape latency were reduced in group MA and EA (P < 0.01, P < 0.05)and the swimming distance were shortened (all P < 0.05). The immunohistochemical DAB staining showed: Comparedwith group C, the average optical density of GFAP in the hippocampal CA1 area of group M increased significantly (P <0.01); the optical density of GFAP in CA1 area of hippocampus in both groups MA and EA was significantly decreased(all P < 0.01), while the optical density of GFAP in MA group was lower than that of EA group (P < 0.05). Western blottest showed: Compared with group C, the expression of GFAP protein obviously increased (P < 0.01). Compared withgroup M, the expression of GFAP protein obviously decreased and had a downregulation tendency in group MA and EA(all P < 0.05), while the expression of GFAP protein decreased in MA group compare with EA group (P < 0.05).Conclusion: Both music electro-acupuncture and pulsed electro-acupuncture can improve the learning and memoryabilities of SAMP8 mice and decrease the expression of GFAP, which may be related to its mechanism of reducingneuroinflammatory reaction, improving apoptosis and eventually protecting neurons, and music electro-acupuncture hasa better tendency than pulsed electro-acupuncture.

国家自然科学基金委青年基金项目（81603678）：督脉电针治疗AD双转基因小鼠不同脑区神经元凋亡作用机制的研究，负责人：唐银杉；国家自然科学基金委青年基金项目（81503654）：逆针灸对老年性痴呆小鼠“抗衰老基因”Klotho的调控，负责人：许安萍。