目的：研究清肠温中方对葡聚糖硫酸钠（DSS）诱导溃疡性结肠炎（UC）大鼠肠黏膜屏障的影响。方法：采用4.5%DSS溶液制备大鼠UC模型，给予清肠温中方及美沙拉嗪灌胃，Western blot、Real time-PCR检测结肠claudin-1、claudin-4的表达；应用免疫组织化学法检测NF-κB p65蛋白的表达及分布。结果：与模型组相比，清肠温中方和美沙拉嗪组大鼠结肠claudin-1、claudin-4的基因和蛋白表达水平均显著升高（P < 0.05），NF-κB p65平均光密度显著降低（P < 0.05）。结论：清肠温中方可能通过下调NF-κB p65的表达，促进claudin-l、claudin-4的表达，修复肠粘膜损伤，最终达到治疗UC的目的。

Objective: The purpose of this paper is to study the effect of Qingchang Wenzhong Decoction on intestinal mucosal barrier in the rats with DSS-induced ulcerative colitis (UC) Methods: the UC model was made using 4.5% (w/v)DSS liquor. Meanwhile, the gavage of Qingchang Wenzhong decoction and mesalazine was given. Claudin-1 and claudin-4 expression were detected by Western blot and Real time-PCR. The expression and distribution of NF-κB p65 proteinwere detected by immunohistochemistry.Results: compared with the model group, the genes and protein levels of claudinland claudin-4 of colitis rats with Qingchang Wenzhong decoction and mesalazine group significantly increased (P <0.05), and the average optical density of NF-κB p65 significantly decreased（P < 0.05）Conclusion: Qingchang Wenzhongdecoction could promote the expression claudin-l and claudin-4 by reducing the expression of NF-κB p65, repairingintestinal mucosal damage and finally treating UC.

北京中医药大学青年教师项目（2018-JYBZZ-JS097）：基于肠道菌群调控肠上皮细胞TLR4/Blimp-1/NLRP12功能轴研究清肠温中方重建溃疡性结肠炎“肠道微生态-宿主免疫”平衡的机制研究，负责人：毛堂友；国家自然科学基金委青年基金项目（81403369）：清肠温中方对溃疡性结肠炎大鼠MSP-RON通路的调控机制研究，负责人：史瑞；北京市科学技术委员会科技计划项目(Z151100003815011)：十病十药研发——清肠温中方治疗溃疡性结肠炎的中药制剂的开发研究，负责人：李军祥。