目的：明确甘草非药用部位总黄酮、总皂苷的积累动态及外源硅对其非药用部位总黄酮、总皂苷积累的影响效应，为甘草药用资源的综合利用提供理论依据。方法：采用盆栽试验栽培甘草植株，在其生长周 期内动态采集甘草非药用部位，采用UV-Vis法测定甘草非药用部位中总黄酮、总皂苷含量。结果：甘草叶中总 黄酮、总皂苷含量显著高于茎中。甘草茎中总黄酮含量在生长周期呈“V”型变化规律，而叶中总黄酮含量呈倒 “V”型。不同浓度外源硅明显提高了不同采样时期甘草茎、叶中总黄酮、总皂苷含量，且这种促进效应的大小 因采样时期、采样部位和硅浓度不同而异。综合而言，0.6 g/kg外源硅对总黄酮、总皂苷积累的促进作用最强。 结论：硅对盐胁迫下甘草非药用部位茎、叶中总黄酮、总皂苷的积累有明显的促进作用。

Objective: To systematically study the accumulation of total saponins in non-medicinal parts of Glycyrrhiza uralensis Fisch. (G. uralensis) and the effects of silicon on the accumulation of total flavonoids and total saponins in nonmedicinal parts of G. uralensis, and to provide a scientific basis for the comprehensive utilization of G. uralensis resources. Methods: G. uralensis were cultivated in pot experiment. The content of total saponins and total flavonoids in non- medicinal parts of G. uralensis by means of UV- visible spectrophotometer was determined by dynamically collecting the non- medicinal parts of G. uralensis. Results: The content of total flavonoids and total saponin of G. uralensis leaves were significantly higher than the stem. The content of total flavonoids in G. uralensis stems showed "V" pattern in growth period, and the content of total flavonoids in leaves was "V". Different concentrations of exogenous Si significantly increased the contents of total flavonoids and total saponins in stems and leaves of G. uralensis at different sampling times, and the magnitude of this promotion was different according to sampling period, sampling site and Si concentration. Taken together, a silicon concentration of 0.6 g/kg had the strongest effect on the accumulation of total flavonoids and total saponins. Conclusion: Silicon could significantly promote the accumulation of total flavonoids and total saponins in stems and leaves of non-medicinal parts of G. uralensis under salt stress.

国家自然科学基金委地区项目（31260304）：硅提高盐胁迫下甘草药材产量和甘草酸含量的潜力及机制，负责人：张新慧；国家自然科学基金委 地区项目（31460330）：旱盐逆境下硅提高甘草综合水分利用效率的生理生态机制，负责人：张新慧；国家科技部重点研发中医药现代化课题六 （2017YFC1700706）：基于生理生态的中药材（甘草）生态农业研究与应用，负责人：张新慧。